Abstract

This study aimed to evaluate the efficacy of magnetic nanocomposite of cefotax against MRSA. A total of 190 samples were collected from milk, farm personnel and different environmental components from the dairy farm under the study to isolate S. aureus. Cefotax based magnetic nanoparticles was synthetized by the adsorption method and marked using Fourier-transform infrared spectrum (FT-IR), and X-ray diffraction (XRD), then it was characterized using Scanning and Transmission Electron Microscope (SEM and TEM). The obtained results revealed that number of positive samples of S. aureus isolation were 63 (33.1%), mainly from feed manger followed by milk machine swabs (60.0 and 53.3%, respectively) at X2 = 48.83 and P < 0.001. Obtained isolates were identified biochemically and by using molecular assays (PCR), also mec A gene responsible for resistance to cefotax was detected. Testing the sensitivity of 63 isolates of S. aureus showed variable degree of resistance to different tested antibiotics and significant sensitivity to cefotax based magnetic nanoparticles at P < 0.05. It was concluded that dairy environment might act a potential source for transmission of MRSA between human and animal populations. In addition, cefotax based magnetic nanoparticles verified an extreme antimicrobial efficacy against MRSA.

Details

Title
Cefotax-magnetic nanoparticles as an alternative approach to control Methicillin-Resistant Staphylococcus aureus (MRSA) from different sources
Author
Mohamed Manar Bahaa El Din 1 ; Abo El-Ela Fatma I 2 ; Mahmoud, Rehab K 3 ; Farghali, Ahmed A 4 ; Gamil Shymaa 4 ; Aziz Sahar Abdel Aleem Abdel 1 

 Beni-Suef University, Department of Hygiene, Zoonoses and Epidemiology, Faculty of Veterinary Medicine, Beni-Suef, Egypt (GRID:grid.411662.6) (ISNI:0000 0004 0412 4932) 
 Beni-Suef University, Associate professor of Pharmacology, Department of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef, Egypt (GRID:grid.411662.6) (ISNI:0000 0004 0412 4932) 
 Beni-Suef University, Department of Chemistry, Faculty of Science, Beni-Suef, Egypt (GRID:grid.411662.6) (ISNI:0000 0004 0412 4932) 
 Beni-Suef University, Department of Materials Science and Nanotechnology, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef, Egypt (GRID:grid.411662.6) (ISNI:0000 0004 0412 4932) 
Publication year
2022
Publication date
2022
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2619056224
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.