Abstract

Folliculogenesis is a complex biological process involving a central oocyte and its surrounding somatic cells. Three-dimensional chromatin architecture is an important transcription regulator; however, little is known about its dynamics and role in transcriptional regulation of granulosa cells during chicken folliculogenesis. We investigate the transcriptomic dynamics of chicken granulosa cells over ten follicular stages and assess the chromatin architecture dynamics and how it influences gene expression in granulosa cells at three key stages: the prehierarchical small white follicles, the first largest preovulatory follicles, and the postovulatory follicles. Our results demonstrate the consistency between the global reprogramming of chromatin architecture and the transcriptomic divergence during folliculogenesis, providing ample evidence for compartmentalization rearrangement, variable organization of topologically associating domains, and rewiring of the long-range interaction between promoter and enhancers. These results provide key insights into avian reproductive biology and provide a foundational dataset for the future in-depth functional characterization of granulosa cells.

The domestic chicken Gallus gallus domesticus is a classic model for the study of folliculogenesis. Here the authors integrate multi-omics analyses characterizing the dynamic transcriptome and chromatin architecture in granulosa cells during chicken folliculogenesis.

Details

Title
Dynamic transcriptome and chromatin architecture in granulosa cells during chicken folliculogenesis
Author
Li, Diyan 1   VIAFID ORCID Logo  ; Chunyou, Ning 1 ; Zhang Jiaman 1 ; Wang, Yujie 1   VIAFID ORCID Logo  ; Tang Qianzi 1   VIAFID ORCID Logo  ; Hua, Kui 1   VIAFID ORCID Logo  ; Wang, Tao 1 ; He Mengnan 1 ; Long, Jin 1   VIAFID ORCID Logo  ; Li, Jing 1 ; Lin, Yu 1 ; Zeng, Bo 1 ; Yin Huadong 1 ; Zhao, Xiaoling 1 ; Zhang, Yao 1 ; Xu Huailiang 2 ; Zhu, Qing 1 ; Li, Mingzhou 1   VIAFID ORCID Logo 

 Sichuan Agricultural University, Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Chengdu, China (GRID:grid.80510.3c) (ISNI:0000 0001 0185 3134) 
 Sichuan Agricultural University, College of Life Science, Ya’an, China (GRID:grid.80510.3c) (ISNI:0000 0001 0185 3134) 
Publication year
2022
Publication date
2022
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2619582080
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.