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Abstract
The prebiotic properties of xylooligosaccharides (XOS) and arabino-xylooligosaccharides (AXOS) produced from rice husk (RH) using microwave treatment combined with enzymatic hydrolysis were evaluated. The RH was subjected to microwave pretreatment at 140, 160 and 180 °C for 5, 10 and 15 min to obtain crude arabinoxylan (AX). Increasing microwave pretreatment time increased sugar content. Crude AX was extracted with 2% (w/v) sodium hydroxide at 25 °C for 24 h and used as a substrate for XOS production by commercial xylanases. Results showed that oligosaccharides produced by Pentopan Mono BG and Ultraflo Max provided xylobiose and xylotriose as the main products. AXOS was also present in the oligosaccharides that promoted growth of Lactobacillus spp. and resisted degradation by over 70% after exposure to simulated human digestion.
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1 Chiang Mai University, Faculty of Agro-Industry, Chiang Mai, Thailand (GRID:grid.7132.7) (ISNI:0000 0000 9039 7662); Chiang Mai University, Cluster of High Value Products from Thai Rice and Plants for Health, Chiang Mai, Thailand (GRID:grid.7132.7) (ISNI:0000 0000 9039 7662); Chiang Mai University, Cluster of Innovative Food and Agro-Industry, Chiang Mai, Thailand (GRID:grid.7132.7) (ISNI:0000 0000 9039 7662)
2 Saga University, Graduate School of Agriculture, Honjo, Saga, Japan (GRID:grid.412339.e) (ISNI:0000 0001 1172 4459)
3 Chiang Mai University, Faculty of Agro-Industry, Chiang Mai, Thailand (GRID:grid.7132.7) (ISNI:0000 0000 9039 7662); Chiang Mai University, Research Center for Development of Local Lanna Rice and Rice Products, Chiang Mai, Thailand (GRID:grid.7132.7) (ISNI:0000 0000 9039 7662)
4 Chiang Mai University, Faculty of Agro-Industry, Chiang Mai, Thailand (GRID:grid.7132.7) (ISNI:0000 0000 9039 7662); Chiang Mai University, Cluster of Innovative Food and Agro-Industry, Chiang Mai, Thailand (GRID:grid.7132.7) (ISNI:0000 0000 9039 7662)
5 Naresuan University, Department of Agro-Industry, Faculty of Agriculture Natural Resources and Environment, Phitsanulok, Thailand (GRID:grid.412029.c) (ISNI:0000 0000 9211 2704)