1. Introduction
Jasmonates (JAs), are lipid-derived natural phytohormones that act as ubiquitous moderators of a broad range of biological processes including plant growth [1,2,3,4,5,6,7], defense against pathogen infection, herbivorous insect attack, and abiotic stress [8,9,10,11]. JAs are also active in specialized metabolism through programing the transcripts of JA-responsive genes across the plant kingdom, from gymnosperms to angiosperms [12,13,14,15,16,17], implying that the usage of JAs or JA-associated networks may be a valuable strategy for future plant improvement.
JA biosynthesis has been deeply explored in dicotyledonous and monocotyledonous plants such as Arabidopsis and rice [5]. These results indicate that JA biosynthesis is initiated by a series of sequential oxygenations of α-linolenic acid using lipoxygenase (LOX), allene oxide synthase (AOS), and allene oxide cyclase (AOC), leading to the synthesis of 12-oxo-phytodienoic acid (OPDA). OPDA is then catalyzed and converted to (+)-7-iso-JA by an OPDA reductase 3 (OPR3) and three rounds of β-oxidation; thus, the above de novo JA biosynthesis is well known as the octadecanoid pathway [18]. Afterwards, the studies found that (+)-7-iso-Jasmonoyl-L-isoleucine (JA-Ile), synthesized from (+)-7-iso-JA by jasmonate amino acid synthetase 1 (JAR1), is considered the major form of active JA in plants [18,19].
In addition, substantial research has found that JA perception and signal transduction, as well as transcript reprogramming of downstream genes, are a delicate and dynamic process containing numerous regulators [20,21]. In Arabidopsis, under normal conditions with a lower JA level, a series of JASMONATE ZINC-FINGER INFLORESCENCE MERISTEM (ZIM)-DOMAIN (JAZ) proteins act as transcriptional repressors of JA signaling. JAZ function to recruit TOPLESS (TPL) or TPL-related proteins (TPRs) by interacting with the adaptor protein, Novel Interactor of JAZ (NINJA), which together form the co-repressor complex which represses the transcriptional regulatory activity of transcription factors (TFs) [22]. This regulatory process suppresses the expression of JA-responsive genes and attenuates various downstream JA-mediated responses. In contrast, the activity of JAZ proteins is delicately controlled by the JA receptor, CORONATINE INSENSITIVE 1 (COI1) [23], an F-box protein which is a component of the Skp1-Cullin-F-box (SCF) E3 ubiquitin ligase complex (SCFCOI1). Under stress stimuli, JA and Ile conjugate to form the bioactive JA-Ile, which is then recognized by COI1, forming the COI1-Ile complex, which interacts with JAZ repressors. This interaction then triggers the ubiquitination and subsequent degradation of the JAZ repressors by the action of the SCFCOI1 complex and the 26S proteasome [19], which releases the TFs to regulate the transcripts of diverse JA-responsive genes involved in JA-regulated biological processes [4,15,24]. Subsequently, numerous studies have uncovered that the multifaceted function of JA is highly attributable to the pairwise interactions of the different TFs and JAZ repressors which regulate the transcription of target genes in a temporal and spatial-dependent manner in plants [4,8,14,25,26]. In addition, it was reported that JA has synergistic or antagonistic functions with abscisic acid (ABA), gibberellic acid (GA), ethylene (ETH), and salicylic acid (SA) in controlling plant growth and stress responses. Notably, JAZ proteins often act as an important hub for regulating the crosstalk of JA and other hormones to dynamically adjust growth, and accurately cope with environmental stresses [27,28,29,30]. Thus, JAZ repressors are not only a crucial link in the COI1-JAZ-TFs module that regulates paths of JA signal transduction, but also a major regulator that integrates multiple signaling pathways.
The JAZ protein family is known as a member of the plant-specific TIFY superfamily, which contains TIFY, ZIM-like (ZML), PEAPOD (PPD), and JAZ protein subfamilies [31]. Of these, JAZ proteins have a conserved TIFY (also known as ZIM) domain containing a core TIF[F/Y]XG motif near the N-terminal region of JAZ protein sequences, and a JA-associated (also named CCT_2) domain which contains the extremely conserved sequence “SLX2FX2KRX2RX5PY”, located in the C-terminus [31,32,33]. In fact, the TIFY domain is not only necessary for JAZ proteins to recruit transcriptional co-repressors like TPL or TPRs via interacting with the NINJA protein [22], but also functions to mediate the paired interactions of JAZ proteins to form homo- and heterodimers [34]. The Jas domain, on the other hand, mediates the JA-Ile dependent interaction of JAZ protein with COI1, or the interaction of JAZ proteins with various TFs [30]. Interestingly, several JAZ proteins, like Arabidopsis AtJAZ5-AtJAZ8 and AtJAZ13 also have additional ethylene-responsive elements binding factor-associated amphiphilic repression (EAR) motifs that can recruit TPL/TPRs in the absence of NINJA [35,36], thus providing additional functional paths for JAZ proteins in plants.
JAZ proteins were first identified in Arabidopsis thaliana in 2007 [19], and are widely present in the green plant lineage. For instance, there are 13, 34, 15, 18 and 13 JAZ proteins identified in A. thaliana [33], Triticum aestivum [37], Oryza sativa [38], Hevea brasiliensis [39], and Solanum lycopersicum [40]. Next, functional studies indicated that different JAZ genes have varied biological function. Arabidopsis AtJAZ4 is involved in regulating the development of root, hypocotyl, and petiole [41], and AtJAZ proteins promote growth and reproductive success partially by attenuating the abnormal metabolic effects of an unrestrained immune response [42]. Likewise, MpJAZ, a homolog of AtJAZ, regulated cell growth and reproductive fitness in Marchantia polymorpha [43]. In addition, overexpression of VqJAZ7 enhanced resistance to powdery mildew by controlling cell death and the accumulation of superoxide anions [44]. TaJAZ1 positively regulated powdery mildew resistance via up-regulating expression of defense-related gene TaPR1/2 and increasing reactive oxygen species accumulation in wheat [45]. In rice, up- or down-regulation of OsJAZ1 or OsJAZ9 resulted in drought response or salt sensitivity [46,47]. It has also been reported that Glycine soja GsJAZ2 positively regulate salt and alkali stress tolerance [48]. More importantly, several lines of evidence have revealed the key role of JAZ proteins in biosynthesis of plant specialized metabolism such as alkaloids, artemisinin, and tanshinone. In Artemisia annua, AaJAZ8 negatively regulated the biosynthesis of artemisinin, a first line of defense against malaria, in response to JA elicitation [24]. In Salvia miltiorrhiza, overexpression of SmJAZ3 and SmJAZ9 reduced tanshinone content [49]. Therefore, JAZ genes have a broad role in regulating adaptability to environmental challenges, and modulate development and specialized metabolism in different plants.
Mentha canadensis L. belongs to the Lamiaceae family, is a widely cultivated aromatic plant, and is also a common medicinal plant in China [50,51]. Furthermore, M. canadensis and its relatives are a source of high-value natural products including essential oils (EOs) widely used in cosmetics, aromatherapy industries, and also exhibit natural antioxidant properties, antibacterial properties, and pharmaceutical value [52,53,54]. These species are also promising bioenergy feedstocks for ethanol production [50,55] or use as natural insecticides for pest management in agriculture [56]. Meanwhile, pioneering studies have found that monoterpenes including (−)-Limonene, (−)-Carvone, (+)-Menthofuran, (−)-Menthone, and (−)-Menthol, a kind of C10 class of terpenoid, are the major constituents of EOs in mint. The de novo biosynthetic pathway of monoterpenoid EOs derived from the upstream methylerythritol phosphate (MEP) pathway has been systematically discovered in the two reported mint cultivars peppermint (Mentha × piperita L.) and spearmint (Mentha spicata L.) in a context-dependent manner. Meanwhile, research into the physiology and molecular biology of these compounds uncovered that the yield and composition of EOs is varied among the mint family in response to SA [57], JA [58], GA [59], high temperatures [53], salinity and copper stress [60], and this phenomenon may be associated with transcriptional regulation and epigenetic modification of EO biosynthetic enzyme genes, and the catalytic properties of a series of EO biosynthetic enzymes [61,62,63].
By contrast, previous genetic studies of M. canadensis are scant due to a lack of sequence data and mainly focused on chemical composition and medicinal usage [64], plant growth and physiological assays [65], and the functional analysis of putative EO biosynthetic enzyme genes including the Geranyl diphosphate synthase subunit (GPPS), and Limonene synthase (LS) [66]. However, little is known about gene families that are closely associated with EO biosynthesis in M. canadensis. Recently, transcriptome sequencing of M. canadensis under Methyl jasmonate (MeJA) treatment uncovered that JA was involved in promoting EO biosynthesis [67], similar to earlier reports [58], and the release of transcriptome data of M. canadensis provides basic information to analyze the key genes involved in regulating different aspects of M. canadensis including EO biosynthesis. As mentioned, JAZ proteins play an important role in JA signaling and JA-mediated secondary metabolism. Thus, based on the reported transcriptome sequencing data of M. canadensis, we first identified McJAZ family genes, analyzed their sequence properties, structural features, phylogenetic relationships, amino acid composition, and expression profiles in different tissues or under JA, ABA, and abiotic stress treatments. In addition, our results showed that McJAZ proteins were localized to the nucleus or cytoplasm, and most of them interacted with McCOI1a in a coronatine-dependent manner, and also formed homo- or heterodimers in M. canadensis. Thus, the current results enrich our knowledge of JAZ family genes and provide a valuable foundation for functional analysis and exploitation of the candidate McJAZ genes for genetic improvement of M. canadensis.
2. Results
2.1. Identification and Molecular Cloning of McJAZ Genes from M. canadensis
To identify the putative McJAZ family genes in M. canadensis, in total, twelve well-known JAZ protein sequences from A. thaliana [19] and nine reported JAZ protein sequences from A. annua [24] were collected and used as queries to perform a BLASTP search based on the previous transcriptome data (SRP132644) at the National Center for Biotechnology Information (NCBI) [67]. Finally, we identified and annotated eight non-redundant full-length McJAZ genes in the M. canadensis transcriptome database. Next, we obtained seven amplicons corresponding to seven distinct full-length McJAZ genes through PCR amplification with gene specific primers. Subsequently, the purified amplicons were ligated with pClone007 Blunt Simple vector, and the resulting products were transformed into Escherichia coli DH5α and sequenced, respectively. After this, we successfully cloned seven full-length McJAZ genes, and denoted them as McJAZ1-McJAZ7 (Figure 1 and Table 1). As expected, all of these McJAZ proteins contained a N-terminus conserved TIFY domain and a C-terminus Jas domain (also named CCT_2 motif). These findings were further corroborated by using the online SMART tools (
Next, sequence analysis showed that the length of the McJAZ genes coding sequences (CDs) ranged from 372 (McJAZ7) to 1041 (McJAZ3) base pairs (bp), which was similar in length to the known AtJAZ CDs ranging from 396 (AtJAZ8) to 1059 (AtJAZ3) bp in A. thaliana [19]. Furthermore, these candidate McJAZ genes encoded predicted products which varied in length from 123 (McJAZ7) to 346 (McJAZ3) amino acids (aa) residues, with molecular weights (MW) ranging from 13.87 kilodalton (kDa) (McJAZ7) to 36.89 kDa (McJAZ3), and the isoelectric point (pI) ranged from 6.12 (McJAZ2) to 9.96 (McJAZ6) (Table 1). Notably, the pI features of most of McJAZ proteins except for McJAZ2 were greater than 7, indicating that most of the McJAZ proteins were basic proteins. Taken together, all of these results provide an earlier basis for further characterization of JAZ family proteins or related networks in M. canadensis.
2.2. Sequence Alignment and Phylogenetic Analysis of McJAZ Genes
To understand the evolutionary and phylogenetic relationships of the JAZ family genes between M. canadensis and other known species including A. thaliana, O. sativa, and A. annua, a phylogenetic tree was constructed based on an unrooted neighbor-joining (NJ) method using twelve AtJAZ proteins from A. thaliana, fifteen OsJAZ proteins from O. sativa, nine AaJAZ proteins from A. annua, and seven McJAZ proteins from M. canadensis. The results showed that all the JAZ proteins were clustered into four groups (Groups A–D) and further classified into A1, A2, B1–B3, C, and D subgroups according to the reported phylogenetic analysis of AtJAZ proteins in Arabidopsis [34]. Among them, all McJAZ proteins were distributed into 5 subgroups, such that subgroup A1 contains McJAZ5, subgroup A2 contains McJAZ3 and McJAZ4, subgroups B1 and B2 contain McJAZ1 and McJAZ2 respectively, and McJAZ6, McJAZ7 belongs to subgroup D (Figure 3).
To analyze the similarity of the homologous sequences among the candidate McJAZ proteins, multiple sequence alignment analysis of the seven McJAZ proteins or the pairwise McJAZ proteins was carried out. The results showed that except for McJAZ6 and McJAZ7, which shared 80.80% identity at the amino acid sequence level (Supplemental Table S1), the rest of pairwise McJAZ proteins contained differential amino acid identity ranging from 6.94% to 27.80% shared identity, analyzed by local DNAMAN 6.0 software (Figure 2A and Supplemental Table S1). In contrast to the sequences overall, the typical TIFY and Jas domains were conserved in all McJAZ proteins in M. canadensis (Figure 2A). Similarly, the yield sequence logos drawn using the MEME tool also showed highly conserved amino acid sequence within the TIFY and Jas domains (Figure 2B). In addition, we also found that McJAZ6 and McJAZ7 possessed the typical EAR (LELRL) motif in the N-terminal region that is widely present in JAZ proteins across different plants (Figure 2A). Interestingly, evidence suggests that the TIFY or Jas motif is responsible for mediating protein-protein interactions for itself or other partners [19,34]. Overall, these results showed the possibility that the function of the seven McJAZ proteins may be conserved within M. canadensis.
2.3. Tissue Expression Patterns of McJAZ Genes
To further investigate expression patterns of McJAZ genes in M. canadensis, we analyzed the expression levels of each McJAZ gene across different tissues (Figure 4A) including roots, stems, young leaves, and flowers using quantitative real-time PCR (qRT-PCR) assays. The results showed that all McJAZ genes were broadly expressed in all evaluated tissues and displayed the highest expression in flowers (Figure 4). More specifically, expression patterns fluctuated across tissues and were significantly different for each McJAZ gene and could be classified into three types. As shown in Figure 4B, the expression profiles of McJAZ1, McJAZ2, McJAZ4, McJAZ5, and McJAZ6 showed similar expression patterns, with the highest levels in flowers, followed by young leaves, stems, and relatively low expression in roots compared with other tissues. In addition, McJAZ3 was found to be most highly expressed in flowers, and its expression was successively reduced in young leaves, roots, and stems. We next found that McJAZ7 showed highest expression in flowers, moderately expression in stems and young leaves, and low expression in roots (Figure 4B). These results suggest that the seven McJAZ genes studied here were constitutively expressed in all four M. canadensis tissues, and most of them were highly abundant in the flower and young leaf tissues.
2.4. Expression Profiles of McJAZ Genes in Response to JA and ABA Treatments
Numerous reports have shown that MeJA and ABA play a vital role in regulating plant development, stress response, and various physiological processes through controlling the expression patterns of downstream genes [8,18,68,69]. Hence, to next investigate the potential roles of McJAZ genes in response to MeJA and ABA, qRT-PCR was performed to analyze the relative expression profiles of these McJAZ genes in M. canadensis that had been treated with MeJA or ABA. Based on qRT-PCR results, we found that the expression pattern of each McJAZ gene was variable under MeJA or ABA treatment (Figure 5). Under MeJA treatment, the transcript levels of seven McJAZ genes were distinctly up-regulated (Figure 5A), similar to previously reported transcriptome data (Supplemental Figure S1) [67]. In general, McJAZ1, McJAZ2, McJAZ6, and McJAZ7 transcript levels displayed a rapid increase, peaking at 1 h after MeJA exposure, and then gradually decreased at 3, 6, and 12 h, and returned to approximately control levels (CK 0 h) after prolonged MeJA exposure (24 h). In contrast, McJAZ3, McJAZ4, and McJAZ5 expression increased at 1 h after MeJA exposure, and significantly increased until a peak after 3 h, then gradually decreased after prolonged MeJA exposure (6, 12, and 24 h) (Figure 5A).
In addition, we found that except for McJAZ4, the transcripts of McJAZ genes were also dynamically affected by exogenous ABA treatment. Among them, McJAZ2, McJAZ5, and McJAZ6 transcript levels were significantly up-regulated at 1, 3, and 24 h for McJAZ2, at 1, 12, and 24 h for McJAZ5, and at 1 and 24 h for McJAZ6 after ABA exposure (Figure 5B). In particular, the expression levels of McJAZ2, McJAZ5, and McJAZ6 reached their highest levels at 14, 21, and 7 times higher than the control (CK 0 h) at 1 h respectively. Additionally, the expression of McJAZ1 was slightly up-regulated at 1 h, but down-regulated subsequently (3, 6, 12, and 24 h), and reached its lowest level at 6 h after ABA exposure. The transcript level of McJAZ3 was not obvious different at 1, 3, and 24 h, but was down-regulated at 6 and 12 h, compared with control (CK 0 h). McJAZ7 expression was slightly decreased after 1, 3, 6, and 12 h of ABA exposure, and then returned to a level comparable to that seen at the control time point (CK 0 h). These results revealed that McJAZ genes expression responds to JA and ABA, and may be involved in JA or ABA signaling pathway in M. canadensis.
2.5. McJAZ Genes Are Involved in Response to Drought and Salt Stress
Abiotic stress is known to severely limit plant growth and development, and affect multiple physiological processes, and the species’ geographical distribution. To further examine the potential functions of McJAZ genes in response to abiotic stresses, qRT-PCR assays were employed to investigate the expression patterns of McJAZ genes in M. canadensis treated with drought and salt stress. The results showed that, under drought stress treatment, the expression levels of the seven McJAZ genes were significantly up-regulated at the individual time point, and expression levels varied by gene (Figure 6A). Expression of McJAZ1, McJAZ2, and McJAZ7 or McJAZ3 reached maximum levels that were 2 to 37 times higher than the control at 12 h or 1 h. In addition, McJAZ4 expression was gradually induced by drought and peaked at 12 h, later declining to a level similar to the control (CK 0 h) at 24 h after drought treatment. On the other hand, the transcripts of McJAZ5 and McJAZ6 were also gradually induced, and reached their highest levels at nearly 8-fold and 39-fold at 24 h under drought treatment compared with control (Figure 6A).
In addition, seven McJAZ genes had a fluctuating and significantly different transcriptional response to salt treatment (Figure 6B). Among them, McJAZ1 and McJAZ4 expression were down-regulated by salt treatment at a subset of time points compared with control (CK 0 h) (Figure 6B). McJAZ3 expression was generally down-regulated to varying degrees at each time point after salt treatment, and reached the lowest levels at 24 h. Expression analysis of McJAZ genes also showed that McJAZ2 and McJAZ7 expression were up-regulated at 1–12 h, and reached maximum levels of more than 6-fold or 4-fold compared with control (CK 0 h) at 12 h or 6 h, respectively. In addition, the transcript levels of McJAZ5 and McJAZ6 were up-regulated, and peaked at more than 7-fold or 9-fold compared with the control at 3 h, gradually decreased after prolonged salt treatment (6 and 12 h), then returned to levels similar to control (CK 0 h) at 24 h after salt treatment. These results implied that most of the McJAZ genes were sensitive to drought and salt stress treatments, and are likely involved in drought and salt stress related signaling networks in M. canadensis.
2.6. Subcellular Localization of McJAZ Proteins
To further determine subcellular localization of McJAZ proteins, subcellular localization was first predicted using an online tool (
2.7. McJAZ Proteins Interact with McCOI1a
Previous studies report that JAZ proteins play a vital role in JA response through interacting with the JA receptor, CORONATINE INSENSITIVE 1 (COI1) in a JA-Ile- or coronatine (COR, a functional mimic of bioactive JA-Ile)-dependent manner rather than a JA- or MeJA-dependent manner [19,23,71,72]. Some JAZ proteins in non-model plants may also interact with AtCOI1 [71]. To determine the role of McJAZ proteins in the JA response in M. canadensis, we first examined the interaction between AtCOI1 and McJAZ proteins by yeast two-hybrid (Y2H) assays. As shown in Figure 8, all of the yeast transformants grew normally on the control medium (DDO, Figure 8A), whereas none of the McJAZ proteins interacted with AtCOI1 regardless of whether the selection medium (QDO) was supplemented with the JA-Ile mimic, COR (Figure 8B,C). Subsequently, given that protein-protein interaction might occur in a context-dependent manner in different plant species, we next cloned the homologous protein of AtCOI1, designated this as McCOI1a given that other unidentified copies of McCOI1 may exist in M. canadensis, and then examined the interaction between McCOI1a and McJAZ proteins. As expected, interaction was also not detected for McCOI1a and all seven McJAZ proteins under selection medium (QDO) in the absence of COR (Figure 8B). In contrast, except for McJAZ6 and McJAZ7, most of McJAZ proteins including McJAZ1, McJAZ2, McJAZ3, McJAZ4 and McJAZ5 interacted with McCOI1a in selection medium (QDO) supplemented with COR respectively (Figure 8C), suggesting that a conserved interaction between COI1 and JAZ is present in different plant species.
2.8. Homo- and Heterodimeric Interaction of McJAZ Proteins
Our previous results showed that all of the McJAZ proteins have a conserved TIFY domain (Figure 1 and Figure 2), and previous evidence has also demonstrated that the TIFY domain is involved in regulating homo- and heterodimeric interactions among JAZ proteins [34]. Thus, we further examined the possibility that the seven McJAZ proteins could form homo- or heterodimers by testing 49 combinations among the seven McJAZ proteins in the Y2H assays (Figure 9A,B). As expected, McJAZ proteins could indeed form homo- or heterodimeric pairs as evidenced by growth of yeast in selective medium SD/-L/-T/-H/-A. As shown in Figure 9B, three out of the seven McJAZ proteins including McJAZ2, McJAZ3, and McJAZ5 could form homodimers by reciprocal interaction in yeast. Meanwhile, the different heterodimeric interactions were also observed among the seven McJAZ proteins (Figure 9B). For instance, the interactions between McJAZ1 and McJAZ5, McJAZ2 and McJAZ4, McJAZ5 or McJAZ7, McJAZ4 and McJAZ5 were observed in reciprocal transformations in yeast. Furthermore, McJAZ1 only interacted with McJAZ3, McJAZ4 as the prey, and McJAZ2 interacted with McJAZ3, McJAZ6 as the prey. McJAZ3 could interact with McJAZ1, McJAZ2, McJAZ4 and McJAZ5 as the bait rather than the prey. As the bait, McJAZ6 only interacted with McJAZ2, but no interaction was detected using McJAZ6 as the prey. In addition, the rest of combinations including McJAZ7 and other McJAZ proteins failed to form heterodimers, except for a heterodimeric interaction between McJAZ7 and McJAZ2 that was observed regardless of whether McJAZ7 was used as the prey or the bait. These results suggest the existence of homo- or heterodimeric interactions among McJAZ proteins in M. canadensis.
3. Discussion
JAs play diverse functions across the JA signaling pathway in plants [8]. Notably, JAZ proteins, core negative regulators of JA signaling, are widely reported to play a vital role in JA-mediated biological processes including plant growth, defense against pathogen infection, insect attack, abiotic stress, and plant specialized metabolism [20,41,73]. To date, JAZ family proteins have been systematically identified in various plants including A. thaliana [33], T. aestivum [37], H. brasiliensis [39], and L. aurea [70]. However, research regarding the JAZ gene family has not yet been reported in the economically and medicinally important plant species M. canadensis. Based on the available transcriptome data [67], we performed a comprehensive identification of JAZ proteins in M. canadensis and further investigated their sequence characteristics and protein structure, evolutionary relationships, expression profiles, subcellular locations, and protein-protein interactions. This work provided a basis for subsequent functional analysis of JAZ genes to deepen our understanding of JA signaling in the Lamiaceae family, especially in M. canadensis.
We isolated seven McJAZ genes from M. canadensis, and found that the length of CDs in the McJAZ gene family ranged from 372 to 1041 bp (Figure 1 and Table 1), which was in line with the reported CDs length in the AtJAZ gene family [19]. Moreover, most McJAZ proteins were basic proteins with pI values greater than 7, except for McJAZ2 with a pI value of 6.12, which was attributed to a higher proportion of acidic amino acids in McJAZ2 such as 21 aspartic acids and 12 glutamic acids than those in the rest of McJAZ proteins, and this phenomenon was ubiquitous in JAZ proteins of other plants like Camellia sinensis [74]. In general, JAZ protein at least contains one conserved TIFY and a typical Jas domain at its N- and C-terminal, and some JAZ proteins including Arabidopsis AtJAZ7 and AtJAZ8 have an LxLxL type of EAR motif at the N-terminus [33]. Here, although the amino acid similarity among McJAZ proteins is low (Figure 2 and Supplemental Table S1), each identified McJAZ protein contained one conserved N-terminus TIFY and a C-terminus Jas domain, which further indicated evolutionary conservation of these structures. Furthermore, McJAZ6 and McJAZ7 have an additional EAR motif (LxLxL) in the N-terminus (Figure 2). TIFY has dual functions in regulating homo- and heterodimeric interactions among different JAZ proteins, and recruiting TPL/TPRs by interacting with NINJA protein [22]. The Jas domain is responsible for interacting with COI1 and various TFs including the core regulator MYC2 to complete the JA response [19], and is involved in subcellular localizations of JAZ proteins [75]. The EAR motif in some JAZ proteins is used to directly recruit the TPL-coupled inhibitory complex to attenuate JA response in the absence of NINJA [36]. Given that protein function is tightly associated with conserved domain structure, all of these results suggest that these seven McJAZ proteins may share some common strategy to regulate the perception of JA and JA response in M. canadensis, much like JAZ proteins in different plant species. Notably, the Jas domain of the JAZ protein usually contained a conserved sequence (SLX2FX2KRX2RX5PY), but our results showed that McJAZ2 has a truncated Jas motif (DLPIARKNSLARFLEKRRDR) without the X5PY sequence (Figure 2). Previous studies found that some deviation existed in the Jas domain owing to alternative splicing (AS). For example, AtJAZ10.3, a splice variant of AtJAZ10 protein in Arabidopsis, has a truncated Jas motif (DLPIARRKSLQRFLEKRKER) lacking LVSTSPY (X5PY) sequence in the whole Jas domain of AtJAZ10.1 [76]. Thus, whether McJAZ2 is a splice variant like AtJAZ10.3 merits further investigation.
The tissue expression patterns of target genes are closely tied to gene function. Specifically, JAZ family genes were constitutively and differentially expressed in different tissues of various plants such as wheat [37], rubber tree [39], and L. aurea [70]. Furthermore, the widespread expression of JAZ genes in different plant tissues is involved in regulating plant growth and development [1,41]. Here, we found that seven McJAZ genes were constitutively expressed in the tested tissues, and showed a variable expression profile (Figure 4). These results suggest that different McJAZ genes may play an important role in regulating the growth and development of different tissues in M. canadensis. It is intriguing that seven McJAZ genes showed the highest expression in flowers over all other tissues studied (Figure 4). Among these, the transcripts of McJAZ5/6/7 reached 33-fold, 180-fold and 70-fold within flowers relative to root tissue. Moreover, earlier reports showed that JAZ genes played roles in flower and stamen development in Arabidopsis and in rice [1,19]. Thus, McJAZ genes may share similar functions with the above-mentioned JAZ genes in flower development. In addition, we found that McJAZ1/2/4/5/6 showed similar expression patterns (Figure 4B), which, taken with the conservation of TIFY and Jas domains among them, suggests some functional redundancy in the McJAZ1/2/4/5/6 in M. canadensis.
The defense-related hormones JA and ABA induce fluctuation in transcript abundance of JAZ genes in various plants, and this critical transcriptional regulation of JAZ genes is connected with the potential roles of JAZ including JA- and ABA-mediated plant development, defense, and specialized metabolism [19,29,37,38,45,47]. For instance, JA treatment and other environmental elicitors rapidly induce JAZ gene expression, which in turn attenuates response to JA [20]. In addition, OsJAZ1 was induced by ABA and involved in negative regulation of drought tolerance in rice [47]. Here, the seven studied McJAZ genes were significantly upregulated after MeJA treatment compared with control, further pointing to their probable involvement in JA signaling. Of them, McJAZ1, McJAZ3, McJAZ6, and McJAZ7 showed a short-term response to MeJA treatment at the major time points of 1 and 3 h, whereas McJAZ2, McJAZ4, and McJAZ5 showed a continuous response to MeJA treatment from 1 to 24 h (Figure 5A), suggesting that the transcription of McJAZ genes may fall into distinct regulatory pathways. For ABA treatment, only McJAZ4 expression was not affected by ABA treatment, the relative expression levels of McJAZ1, McJAZ3, and McJAZ7 were slightly depressed, and McJAZ2, McJAZ5, and McJAZ6 genes were up-regulated in a time-point dependent manner (Figure 5B). Notably, cross-talk between JA and ABA has been reported in plants [29]. Our study shows that McJAZ2, McJAZ5, and McJAZ6 are also co-induced by MeJA and ABA, indicating that these McJAZ genes may regulate cross-talk between these two phytohormones. In addition, studies show that the JAZ family genes exhibited varied expression in response to one or more abiotic stressors in different plants. JAZ family genes are differentially induced by drought, salt, and low temperature in wheat, rice, Camellia sinensis, and Brassica rapa, and upregulation of these genes is involved in salt and drought tolerance [37,38,74,77]. Here, we found that the identified seven McJAZ genes showed differential upregulation to cope with drought stress, and McJAZ2, McJAZ5, McJAZ6, McJAZ7 showed an intense response to drought treatment (Figure 6A). Furthermore, McJAZ2, McJAZ5, McJAZ6, McJAZ7 were upregulated after salt treatment (Figure 6B), suggesting that McJAZ genes likely played important roles in resistance to one or more abiotic stressors. In addition, abiotic stress-responsive McJAZ genes were simultaneously upregulated by JA and ABA treatments, suggesting that McJAZ genes may respond to different stresses via diverse phytohormone signaling pathways in M. canadensis.
In plants, JAZ proteins participate in JA signaling pathways by interacting with JA receptor COI1 or themselves [19,34]. In our study, some of the McJAZ proteins were found to interact with McCOI1a in a COR-dependent manner (Figure 8). Furthermore, McJAZ proteins showed variable capacities to form homo- or heterodimers via interacting with each other in a COR-independent manner (Figure 9). These results suggest that the functions of McJAZ gene in M. canadensis might be similar to those in Arabidopsis. Previous studies have shown that AtJAZ1 can interact with AtCOI1 or SlCOI1 in the presence of COR [32]. However, the identified McJAZ proteins only interacted with McCOI1a rather than AtCOI1 in the presence of COR, suggesting that regulation of the interactions of JAZ-COI1 may differ across species. In addition, the phylogenetic relationship among the JAZ genes reveals the putative function of them as homologous genes often share similar biological roles. In this study, we found that McJAZ1 and McJAZ2 clustered with AtJAZ1/2, or AtJAZ5/6 into the subgroup B2 or B1. McJAZ3, McJAZ4, and McJAZ5 clustered with AtJAZ3/4 or AtJAZ10 into the subgroup A2 or A1. McJAZ6/7 clustered with AtJAZ7/8 into the group D (Figure 3). It was clear that AtJAZ proteins typically interacted with AtMYC2 to regulate diverse physiological functions including plant metabolism and defense [78,79]. Additionally, AtJAZ3 interacted with AtSPL9 to regulate plant resistance to insect herbivores [80]. AtJAZ1/4/9 interacted with AtICE1/2 to regulate freezing tolerance in Arabidopsis [25]. AtJAZ1/8/11 interacted with and attenuated the transcriptional functions of MYB21 and MYB24 to regulate stamen development [81]. AtJAZ1/2/5/6/8/9/10/11 also regulated JA-mediated anthocyanin biosynthesis and trichome development by interacting with the WD-Repeat/bHLH/MYB complexes in Arabidopsis [14]. Given the functional specificity, diversity, and redundancy of AtJAZ genes in Arabidopsis [82], McJAZ proteins may regulate plant development, defense, and specialized metabolism including EO biosynthesis by interacting with the as-yet unidentified McMYC2 or additional TFs in M. canadensis (Figure 10). Further investigation needs to be conducted to determine the complete regulatory network of McJAZ proteins.
Notably, JA treatment promoted biosynthesis of EO in two Mentha plants [58,67], and EO from Mentha plants could be used to control plant bacterial disease caused by A. tumefaciens in tomato [83], enhance neighboring plants’ resistance to insect herbivores [56], and act as a natural insecticide for control of insects including Tribolium castaneum, Lasioderma serricorne, and Liposcelis bostrychophila adults [84]. This suggests that manipulation of JA signaling in Mentha plants might have dual roles in regulating EO biosynthesis and affecting the anti-insect or anti-pathogen ability of neighboring plants. JAZ proteins, the key components of the JA signaling pathway, acted as important regulators involved in mediating biosynthesis of specialized metabolism such as anthocyanin [14,85], tanshinone [49], and artemisinin [24] through employing the different regulatory mechanisms. However, the detailed molecular mechanisms of JA-mediated EO biosynthesis by McJAZ genes or a McJAZ-related network remain uninvestigated within the mint family. Thus, subsequent research into functional analysis of McJAZ genes or McJAZ-associated regulatory networks in Mentha plants will deepen our understanding of the regulatory mechanisms of JA-mediated EO biosynthesis, and help us to develop efficient strategies for genetic improvement of EO content, and even enhance plant defense against pathogen infection, herbivorous insect attack, and abiotic stress in M. canadensis.
4. Materials and Methods
4.1. Plant Materials and Treatments
M. canadensis was conserved and planted at the Germplasm Nursery in the Institute of Botany, Jiangsu Province, and Chinese Academy of Sciences, Nanjing, Jiangsu Province [67], for use in each assay described here. Cuttings of M. canadensis were propagated by inserting into water until roots emerged, and then planted into plastic pots containing a mixture of nutrient soil and vermiculite (2:1, v/v). For in vitro culture of N. benthamiana, seeds were sown on soil and 10-day-old seedlings were transplanted into plastic pots containing a mixture of nutrient soil and vermiculite (2:1, v/v). Next, M. canadensis and N. benthamiana plants were grown in a light chamber at 23 ± 2 °C with the desired light regime, and a 16-h light/8-h dark photoperiod.
For hormone and salt stress treatments, two-week-old M. canadensis seedlings were sprayed with 100 μM MeJA (Sigma-Aldrich (Shanghai) Trading Co. Ltd., Shanghai, China), 20 μM ABA (Sigma-Aldrich (Shanghai) Trading Co. Ltd., Shanghai, China), and 150 mM NaCl for 1, 3, 6, 12, and 24 hours (h) according to methods adapted from previous studies [12,86]. For drought treatment, two-week-old hydroponically cultured M. canadensis seedlings were exposed to dry filter paper for 1, 3, 6, 12, and 24 h at room temperature according to methods adapted from previous studies [87]. Untreated plants were used as the control (labeled as CK 0 h). Then, leaves of the control and treated seedlings, of matching positions, were collected at the indicated time point and frozen in liquid nitrogen and stored at −80 °C for further analysis.
4.2. Isolation and Cloning of M. canadensis McJAZ Family Genes
The isolation of M. canadensis McJAZ genes were performed as described previously [88]. In short, the transcriptome data (SRP132644) of M. canadensis was downloaded from the National Center for Biotechnology Information (NCBI,
To clone the McJAZ genes, the young leaves of two-week-old M. canadensis were collected for RNA extraction using the reagent Trizol (Cat# 9108, Takara Biomedical Technology (Beijing) Co., Ltd., Beijing, China), and cDNA was synthesized using PrimeScript RT reagent Kit with gDNA Eraser (RR047A, Takara Biomedical Technology (Beijing) Co., Ltd., Beijing, China). The candidate McJAZ genes were amplified using PCR with defined primers (Supplemental Table S2) in a 25 μL volume including 1 μL 5x cDNA template, 0.5 μL of each forward and reverse primer, 5 μL 5x PrimeSTAR DNA Polymerase buffer, 2 μL PrimeSTAR® DNA Polymerase, and 16 μL sterile ddH2O. The amplification procedure was as follows: 95 °C for 10 min followed by 35 cycles of denaturation for 15 s at 95 °C, annealing at designated temperature for 20 s, and extension at 72 °C for 1 min per kilobase (kb). Subsequently, the full-length coding sequences of McJAZ genes were gel-purified using Gel DNA Mini Purification Kit (TSINGKE Biotechnology, Beijing, China), and the purified amplicons were ligated with pClone007 Blunt Simple vector (TSV-007B, TSINGKE Biotechnology, Beijing, China), and then the resulting products were transformed into Escherichia coli DH5α and sequenced in TSINGKE Biotechnology Co., Ltd (TSINGKE Biotechnology, Chengdu, China).
4.3. Sequence Analysis and Phylogenetic Tree Analysis
The sequence and physio-chemical properties of candidate McJAZ proteins were predicted using the online ExPASy server (
Multiple sequence alignment analysis of the seven McJAZ proteins was performed to analyze the characteristics of the conserved sequences using Clustal X2 software with default settings [91]. Meanwhile, multiple sequence alignment of the pairwise McJAZ proteins to identify the amino acid sequence similarity was done using DNAMAN 6.0 (Lynnon Biosoft, Quebec City, QC, Canada) software with default parameters. The frequency of the conserved amino acids at each site within the TIFY and Jas domain was analyzed using online MEME tools (
4.4. Subcellular Localization of McJAZ Proteins
The full-length coding sequences of each McJAZ gene without its stop codon was PCR-amplified using gene-specific primers (Supplemental Table S2), and the amplicons were subcloned into the N-terminal side of the pCAMBIA1300 vector containing the green fluorescent protein (GFP) reporter gene to produce the recombinant fusion construct pCAMBIA1300-McJAZs-GFP under the control of the CaMV 35S promoter. Then the fusion constructs were transformed into Agrobacterium strains GV3101 through the conventional freezing-thawing method, and the GV3101 strains harboring each pCAMBIA1300-McJAZs-GFP or empty pCAMBIA1300-GFP were transiently infiltrated into 5-week-old N. benthamiana leaves according to previous report [24]. GFP signals were detected 2 to 3 days after infiltration using a confocal laser-scanning microscope (Leica TCS SP5-II, Wetzlar, Germany).
4.5. RNA Extraction and Quantitative Real-Time PCR (qRT-PCR) Analysis
To study tissue expression patterns, different tissues of 3-month-old M. canadensis, including roots, stems, young leaves, and flowers were harvested and stored at −80 °C. Total RNA of the different tissues (labeled in Figure 4A) and the aforementioned samples was isolated using the total RNAprep pure Extraction Kit (Tiangen Biotech, Beijing, China) according to the manufacturer’s recommendations. cDNA was synthesized from 1.0 μg total RNA using the PrimeScript 1st Strand cDNA Synthesis Kit (Takara Biomedical Technology (Beijing) Co., Ltd., Beijing, China) according to the manufacturer’s instructions. The qRT-PCR was performed using the SYBR Premix Ex Taq II Kit (Tiangen Biotech, Beijing, China) according to the manufacturer’s instructions and methods previously described [93]. All PCR reactions were performed on at least three replicates under identical conditions. Expression level of each gene was normalized to the expression of β-actin gene (KM044035) used as internal control in the mint family in the previous study [61], and the relative mRNA expression for each gene was calculated using the 2−∆∆Ct method [94]. All primers are listed in Supplemental Table S2.
4.6. Yeast Two-Hybrid (Y2H) Experiments
Yeast two-hybrid assays were used to test the paired physical interactions among McJAZ proteins, or the interactions between McJAZ proteins and AtCOI1, or its homolog McCOI1a. The full-length coding sequences of McJAZ genes were cloned into the prey vector pGADT7 (Activation domain, AD) or bait vector pGBKT7 (Binding domain, BD) (Takara, Japan), respectively, and the full-length coding sequences of McCOI1a and AtCOI1 were amplified using gene-specific primers (Supplemental Table S2), and inserted into the bait vector pGBKT7 (BD). The indicated combinations were co-transformed into the yeast strain AH109 using the lithium acetate method according to the manufacturer’s instructions (Cat# 630440, Takara Biomedical Technology (Beijing) Co., Ltd., Beijing, China), and bait-only or prey-only was tested with empty AD or BD as negative controls.
To investigate protein-protein interactions, the transformed AH109 yeast cells were first cultivated in liquid control medium SD/-Leu/-Trp (DDO) over 24 h, and then 5 μL of each yeast suspension was taken to inoculate the solid control medium SD/-Leu/-Trp (DDO) and selection medium SD/-Leu/-Trp/-Ade/-His (QDO) with or without 35 μM coronatine (COR), a structural and functional mimic of JA-Ile obtained from several strains of Pseudomonas syringae according to previous studies [19,72,95]. The yeast cells were grown at 30 °C for 4 days before imaging.
4.7. Statistical Analysis
All experiments were conducted with at least three biological replicates. Significant differences between the control versus each treatment were analyzed by Student’s t-test using GraphPad Prism 5 software. The values are mean ± standard error (SE) for replicates in each group. * p values ≤ 0.05 or ** p values ≤ 0.01 were considered as significant or extremely significant.
4.8. Accession Numbers
The gene sequences data mentioned in this article can be found in the NCBI database under the accession numbers: McJAZ1 (MZ229995), McJAZ2 (MZ229996), McJAZ3 (MZ229997), McJAZ4 (MZ229998), McJAZ5 (MZ229999), McJAZ6 (MZ230000), McJAZ7 (MZ230001), and AtCOI1 (AT2G39940).
D.-B.X., W.W. and C.-Y.L. conceived and designed the research. D.-B.X., Y.-N.M., T.-F.Q., W.-L.T., X.-W.Q., X.W., R.-C.L., H.-L.F. and Z.-Q.C. performed the experiments, analyzed data, and planted plant materials. D.-B.X., Y.-N.M. wrote the manuscript. D.-B.X., Y.-N.M. and T.-F.Q. contributed equally to the study. All authors discussed the results and commented on the manuscript. All authors have read and agreed to the published version of the manuscript.
This research was supported by Sichuan Science and Technology Program (2020YJ0406), and the National Natural Science Foundation of China (31900256), and the funds of 1000-Talent Program of Sichuan Province to D.-B. Xu. The funding body have no role in the design of the research, analysis of data, and in writing the draft manuscript.
Not applicable.
Not applicable.
All data in the present research are available in the public database as referred in the Material and Method section.
The authors declare no conflict of interest.
MeJA: Methyl Jasmonate; ABA: Abscisic Acid; qRT-PCR: Quantitative Real-Time Polymerase Chain Reaction; Y2H: Yeast Two-Hybrid; GFP: Green Fluorescent Protein.
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Enlarge this image.Figure 1. McJAZ genes and domain architectures of McJAZ proteins in M. canadensis. Shown above are the coding sequences (CDs) of the seven focal McJAZ genes, and the domain architectures of these McJAZ proteins. The McJAZ proteins share a conserved TIFY domain (indicated by a pink box), and Jas domain (indicated by a blue box) with other JAZ homologous proteins in other plant species.
Enlarge this image.Figure 2. Multiple sequence alignment of McJAZ proteins from M. canadensis. (A) Alignment of the amino acid sequences of McJAZ proteins from M. canadensis. The EAR motif is indicated by a red rectangle. The conserved TIFY and Jas domains are outlined with a saffron yellow straight line. (B) The sequence logos of the TIFY and Jas motif are highly conserved across all McJAZ proteins. The height of each stack indicates conservation of the sequence at the labeled positions, and the height of each letter within each stack indicates the relative frequency of the corresponding amino acid.
Enlarge this image.Figure 3. Phylogenetic analysis of JAZ proteins from M. canadensis and other species. JAZ amino acid sequences from M. canadensis were aligned with those from A. thaliana (eudicot), O. sativa (monocot) and A. annua (eudicot) using Clustal X2, and a neighbor-joining (NJ) phylogenetic tree was constructed using MEGA5.1. Bootstrap values indicate the percentage among 2000 replicates. JAZ proteins of M. canadensis were labeled with red font. Mc, M. canadensis. At, A. thaliana, Os, O. sativa, Aa, A. annua. The accession numbers or locus name of each JAZ amino acid sequence shown is as follows: A. thaliana AtJAZ1, NP973862.1, AtJAZ2, AAP13409.1, AtJAZ3, NP001078174, AtJAZ4, AAX55088.1, AtJAZ5, AAO00903.1, AtJAZ6, AAL15195.1, AtJAZ7, AAR24741.1, AtJAZ8, ABG48454.1, AtJAZ9, AAM10238.1, AtJAZ10, NP001154713.1, AtJAZ11, AAU15160.1, AtJAZ12, AAK93690.1; O. sativa OsJAZ1, Os04g55920, OsJAZ2, Os07g05830, OsJAZ3, Os08g33160, OsJAZ4, Os09g23660, OsJAZ5, Os04g32480, OsJAZ6, Os03g28940, OsJAZ7, Os07g42370, OsJAZ8, Os09g26780, OsJAZ9, Os03g08310, OsJAZ10, Os03g08330, OsJAZ11, Os03g08320, OsJAZ12, Os10g25290, OsJAZ13, Os10g25230, OsJAZ14, Os10g25250, OsJAZ15, Os03g27900; A. annua, AaJAZ1, AJK93412.1, AaJAZ2, AJK93413.1, AaJAZ3, AJK93414.1, AaJAZ4, AJK93415.1, AaJAZ5, APR73266.1, AaJAZ6, APR73267.1, AaJAZ7, APR73268.1, AaJAZ8, APR73269.1, AaJAZ9, APR73270.1.
Enlarge this image.Figure 4. Tissue-specific expression patterns of M. canadensis McJAZ genes. (A) The different tissues used for total RNA extraction, including Root (R), Stem (S), Young Leaf (L) and Flower (F). The red arrow indicates the position at which root and leaf tissues were collected. (B) Quantitative real-time (qRT)-PCR analysis of each McJAZ gene expression in the indicated M. canadensis tissues. The expression level of each McJAZ gene was normalized to β-actin gene and relative expression values are compared in root. Data are presented as means ± SE of three independent replicates, and significant differences between the expression level of each McJAZ gene in root tissue versus other tissue was analyzed by Student’s t-test (* p < 0.05 or ** p < 0.01).
Enlarge this image.Figure 5. Expression profiles of McJAZ genes under phytohormone MeJA and ABA treatments. Quantitative real-time (qRT)-PCR analysis of the expression levels of McJAZ1, McJAZ2, McJAZ3, McJAZ4, McJAZ5, McJAZ6, and McJAZ7 in the two-week-old M. canadensis seedlings under 100 μM Methyl Jasmonate (MeJA) (A) or 20 μM Abscisic Acid (ABA) (B) treatments for 0, 1, 3, 6, 12, and 24 h, respectively. The relative expression level of each McJAZ gene was normalized to β-actin gene and compared with CK (0 h) as reference using the 2−∆∆Ct method. Data are presented as means ± SE of three independent replicates from three propagated cuttings. Asterisks indicate significant differences between the expression level of each McJAZ gene in CK (0 h) versus other time points were analyzed by Student’s t-test (* p < 0.05 or ** p < 0.01). CK (0 h) represents an independent control under identical conditions.
Enlarge this image.Figure 6. Expression profiles of McJAZ genes under drought and salt stress treatments. Quantitative real-time (qRT)-PCR analysis of the expression levels of McJAZ1, McJAZ2, McJAZ3, McJAZ4, McJAZ5, McJAZ6, and McJAZ7 in the two-week-old M. canadensis seedlings under drought (A) or 150 mM NaCl (B) treatments for 0, 1, 3, 6, 12, and 24 h. The relative expression level of each McJAZ gene was normalized to β-actin gene and compared with CK (0 h) as reference using the 2−∆∆CT method. Data are presented as means ± SE of three independent replicates from three propagated cuttings. Asterisks indicate significant differences between the expression level of each McJAZ gene in CK (0 h) versus other time points were analyzed by Student’s t-test (* p < 0.05 or ** p < 0.01). CK (0 h) represents an independent control under identical conditions.
Enlarge this image.Figure 7. Subcellular localization analysis of McJAZ proteins in N. benthamiana leaf cells. The GFP-tagged McJAZ fusion proteins and empty green fluorescence protein (GFP) alone were transiently expressed in the epidermal cells of N. benthamiana leaf under the control of the CaMV 35S promoter. GFP fluorescence was detected at two days after infiltration in N. benthamiana leaves by confocal laser microscopy. GFP alone is labeled as GFP control. The red arrow indicates the nucleus within each image. Bar = 20 μm.
Enlarge this image.Figure 8. Yeast two-hybrid interactions between McJAZ proteins and McCOI1a or AtCOI1. The co-transformed yeast cells harboring the indicated plasmid combinations were inoculated in liquid medium SD/-Leu/-Trp (DDO), and then placed onto control medium SD/-Leu/-Trp (DDO) (A), selection medium SD/-Leu/-Trp/-His/-Ade (QDO) plus Methanol (B), and selection medium SD/-Leu/-Trp/-His/-Ade (QDO) containing 35 μM coronatine (COR) (C). The empty pGADT7 (AD) and pGBKT7 (BD) were used as negative controls. These data represent three independent experiments, and representative photos were taken after 4 days of incubation at 30 °C.
Enlarge this image.Figure 9. Yeast two-hybrid interactions among different McJAZ proteins. Yeast cells transformed with different combinations of constructs containing McJAZ proteins fused with the DNA binding domain (BD-McJAZ1, BD-McJAZ2, BD-McJAZ3, BD-McJAZ4, BD-McJAZ5, BD-McJAZ6, and BD-McJAZ7), McJAZ proteins fused with the activation domain (AD-McJAZ1, AD-McJAZ2, AD-McJAZ3, AD-McJAZ4, AD-McJAZ5, AD-McJAZ6, and AD-McJAZ7), and the pGBKT7 (BD) alone, and the pGADT7 (AD) alone were inoculated in liquid medium and then placed on control medium SD/-Leu/-Trp (SD/-L/-T) (A), and selective media SD/-Leu/-Trp/-His/-Ade (SD/-L/-T/-H/-A) (B). The empty vectors (AD and BD) were transformed into yeast and used as negative controls. These data represent three independent experiments, and representative pictures were taken after 4 days of incubation at 30 °C.
Enlarge this image.Figure 10. A proposed working model of how JA signaling module regulates diverse biological processes in response to JA signaling in M. canadensis. A simple model showing the putative role of the JA signaling module in controlling diverse biological processes in M. canadensis. McCOI1, a homolog of the Arabidopsis F-box protein CORONATINE INSENSITIVE 1 (AtCOI1) (Xie et al., 1998), acts as a putative receptor for the active molecule JA-isoleucine (JA-Ile). Introduction of the exogenous elicitor induces the production of JA-Ile, which is perceived by McCOI1 to form the activated McCOI1-JA-Ile complex that subsequently interacts with the repressor, JASMONATE ZIM DOMAIN (JAZ) proteins, and represses its transcriptional inhibitory ability by degradation of McJAZ proteins through the SCFCOI1-dependent 26S proteasome pathway (Chini et al., 2007; Howe et al., 2018). Next, degradation of the McJAZ proteins relieves repression of downstream transcription factors (TFs) including unidentified McMYC2 or other McTFs, which are involved in regulating plant development, plant defense, and essential oil (EO) biosynthesis in M. canadensis. A photograph of normal growing M. canadensis was used as the model; shown at left.
Molecular characteristics of McJAZ genes in M. canadensis.
| Gene Name | Genbank Accession No. 1 | Length CDs 2 | Amino Acids 3 | MW (kDa) 4 | pI 5 | TIFY Motif 6 | Jas Motif (aa) 7 | Protein Localization |
|---|---|---|---|---|---|---|---|---|
| McJAZ1 | MZ229995 | 726 | 241 | 26.30 | 8.98 | TIFYSG | 196–221 | Nuclei |
| McJAZ2 | MZ229996 | 522 | 173 | 18.35 | 6.12 | TIFYGG | 156–173 | Nuclei |
| McJAZ3 | MZ229997 | 1041 | 346 | 36.89 | 9.75 | TIFYNG | 294–319 | Nuclei |
| McJAZ4 | MZ229998 | 789 | 262 | 28.09 | 9.17 | TIFYGG | 230–249 | Nuclei |
| McJAZ5 | MZ229999 | 549 | 182 | 19.93 | 9.76 | TIFYNG | 158–182 | Nuclei |
| McJAZ6 | MZ230000 | 378 | 125 | 14.02 | 9.96 | TIFYNG | 93–121 | Nuclei |
| McJAZ7 | MZ230001 | 372 | 123 | 13.87 | 9.77 | TIFYNG | 91–119 | Nuclei |
1 Genbank Accession number; 2 the length of coding sequences; 3 the length of amino acid sequences; 4 the molecular weight of protein (kDa); 5 the isoelectric point (pI); 6 the core sequence feature of TIFY motif; 7 the position of Jas domain within the amino acid sequences; aa: amino acid.
Supplementary Materials
The following are available online at
References
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; Ya-Nan Ma 2
; Teng-Fei Qin 3 ; Wei-Lin, Tang 4 ; Xi-Wu, Qi 2 ; Wang, Xia 4 ; Rui-Cen Liu 4 ; Hai-Ling, Fang 2 ; Chen, Ze-Qun 2 ; Cheng-Yuan, Liang 2 ; Wu, Wei 4 1 College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China;
2 Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), No. 1 Qianhu Houcun, Zhongshanmen Wai, Nanjing 210014, China;
3 Henan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, China;
4 College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China;