Abstract

The aim of this study was the isolation and molecular characterization of fungi from untreated refinery effluent by using multiple conserved genes. The Fungi isolated were characterized based on PCR amplification and genomic sequencing of the internal transcribed spacer region (ITS), partial β-tubulin (BenA), calmodulin (CaM), and RNA polymerase second large subunit (RPB2) genes, along with morphological characterization. The obtained sequences were subjected to BLAST analysis and the corresponding fungal isolates were assigned species names after comparison with representative sequences available in GenBank. Fifteen (15) Fungi species belonging to four genera of Aspergillus, Penicillium, Fusarium, and Trichoderma with Aspergillus as the predominant genus were identified. Therefore these genes should be used as molecular markers for species level identification of fungi (especially Aspergillus and Penicillium as proven in this study.

Details

Title
Characterization and phylogeny of fungi isolated from industrial wastewater using multiple genes
Author
Ezeonuegbu Blessing Amaka 1   VIAFID ORCID Logo  ; Dauda, Abdullahi Machido 1 ; Whong Clement M Z 1 ; Wisdom, Sohunago Japhet 2 ; Kassem, Hazem S 3 ; Yaro Clement Ameh 4   VIAFID ORCID Logo  ; Hetta, Helal F 5 ; Mostafa-Hedeab Gomaa 6 ; Zouganelis, George D 7 ; Batiha Gaber El-Saber 8 

1  Ahmadu Bello University, Department of Microbiology, Zaria, Nigeria (GRID:grid.411225.1) (ISNI:0000 0004 1937 1493) 
2  Ahmadu Bello University, Department of Botany, Zaria, Nigeria (GRID:grid.411225.1) (ISNI:0000 0004 1937 1493) 
3  King Saud University, Department of Agricultural Extension and Rural Society, College of Food and Agriculture Sciences, Riyadh, Saudi Arabia (GRID:grid.56302.32) (ISNI:0000 0004 1773 5396) 
4  University of Uyo, Department of Animal and Environmental Biology, Uyo, Nigeria (GRID:grid.412960.8) (ISNI:0000 0000 9156 2260) 
5  Assiut University, Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut, Egypt (GRID:grid.252487.e) (ISNI:0000 0000 8632 679X); University of Cincinnati College of Medicine, Department of Internal Medicine, Cincinnati, USA (GRID:grid.24827.3b) (ISNI:0000 0001 2179 9593) 
6  Beni-Suef University, Pharmacology Department, Faculty of Medicine, Beni Suef, Egypt (GRID:grid.411662.6) (ISNI:0000 0004 0412 4932) 
7  University of Derby, Human Sciences Research Centre, Derby, UK (GRID:grid.57686.3a) (ISNI:0000 0001 2232 4004); Novel Global Community Educational Foundation, Hebersham, Australia (GRID:grid.57686.3a) 
8  Damanhour University, Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour, Egypt (GRID:grid.449014.c) (ISNI:0000 0004 0583 5330) 
Publication year
2022
Publication date
2022
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-022-05820-9
ProQuest document ID
2626564712
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.