Abstract

Ureaplasma urealyticum (UU) is commonly present in human reproductive tract, which frequently leads to genital tract infection. Hence, there is an urgent need to develop a rapid detection method for UU. In our study, a real-time fluorescence loop-mediated isothermal amplification (LAMP) assay was developed and evaluated for the detection of UU. Two primers were specifically designed based on the highly conserved regions of ureaseB genes. The reaction was carried out for 60 min in a constant temperature system using Bst DNA polymerase, and the process was monitored by real-time fluorescence signal, while polymerase chain reaction (PCR) was performed simultaneously. In real-time fluorescence LAMP reaction system, positive result was only obtained for UU among 9 bacterial strains, with detection sensitivity of 42 pg/μL (4.2 × 105 CFU/mL), and all 16 clinical samples of UU could be detected. In conclusion, real-time fluorescence LAMP is a simple, sensitive, specific and effective method compared with conventional PCR, which shows great promise in the rapid detection of UU.

Details

Title
Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection of Ureaplasma urealyticum
Author
Jie-Ni, Shen 1 ; Jing-Yi, Ye 2 ; Meng-Xiao, Lao 3 ; Chu-Qiao, Wang 2 ; Dong-Hong, Wu 2 ; Xiao-Ying, Chen 2 ; Li-Hong, Lin 4 ; Wen-Yan, Geng 5 ; Xu-Guang, Guo 6   VIAFID ORCID Logo 

1  The Third Affiliated Hospital of Guangzhou Medical University, Department of Clinical Laboratory Medicine, Guangzhou, China (GRID:grid.417009.b) (ISNI:0000 0004 1758 4591); The Nanshan School of Guangzhou Medical University, Department of Clinical Medicine, Guangzhou, China (GRID:grid.410737.6) (ISNI:0000 0000 8653 1072) 
2  The Nanshan School of Guangzhou Medical University, Department of Clinical Medicine, Guangzhou, China (GRID:grid.410737.6) (ISNI:0000 0000 8653 1072) 
3  Maternal and Child Health Care Hospital of Guangming District, Department of Clinical Laboratory Medicine, Shenzhen, China (GRID:grid.410737.6) 
4  The Third Clinical School of Guangzhou Medical University, Center for Reproduction Medicine, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou, China (GRID:grid.410737.6) (ISNI:0000 0000 8653 1072) 
5  The Third Affiliated Hospital of Guangzhou Medical University, Department of Clinical Laboratory Medicine, Guangzhou, China (GRID:grid.417009.b) (ISNI:0000 0004 1758 4591); The Third Affiliated Hospital of Guangzhou Medical University, Department of Blood Transfusion, Guangzhou, China (GRID:grid.417009.b) (ISNI:0000 0004 1758 4591) 
6  The Third Affiliated Hospital of Guangzhou Medical University, Department of Clinical Laboratory Medicine, Guangzhou, China (GRID:grid.417009.b) (ISNI:0000 0004 1758 4591); The Third Affiliated Hospital of Guangzhou Medical University, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou, China (GRID:grid.417009.b) (ISNI:0000 0004 1758 4591); The Third Affiliated Hospital of Guangzhou Medical University, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China (GRID:grid.417009.b) (ISNI:0000 0004 1758 4591) 
Publication year
2022
Publication date
Dec 2022
Publisher
Springer Nature B.V.
e-ISSN
21910855
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s13568-022-01357-2
ProQuest document ID
2627872462
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.