Abstract
The consumption of equipment and reagents for specimen collection and detection is high, resulting in elevated screening costs. [...]with the increase in the number of samples collected, the testing capabilities of medical institutions have reached a saturation point, hindering the speed and scope of large-scale screenings. According to the RT-PCR kit instructions, the IAT samples were positive in all 19 cases, while in the 10-in-1 simulation, 18 samples were positive, and the result for the remaining cases was uncertain, as it was positive only for ORF1ab. [...]the IAT and the simulated 10-in-1 test showed a high degree of consistency, with a positive coincidence rate of 94.7%. [...]we collected samples to perform a real 10-in-1 test. For PCR, a two-fold dilution increases the Ct value by only one. [...]this dilution can achieve the same detection efficiency as the original, preserving the assay reliability.
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Details
1 Department of Laboratory Medicine, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, China
2 Department of Laboratory Medicine, Dalian Sixth People's Hospital, Dalian, Liaoning 1160311, China
3 Microbiological Laboratory, Dalian Municipal Center for Disease Control and Prevention, Dalian, Liaoning 116019, China
4 Department of Laboratory Medicine, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, China
5 Committee of the Party Group, Health Commission of Dalian, Dalian, Liaoning 116006, China
6 Department of Laboratory Medicine, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, China; Clinical Laboratory and Medical Quality Control Center, Health Service Center of Liaoning Province, Shenyang, Liaoning 110005, China