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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Background: Acid Blue 113 (AB113) is a typical azo dye, and the resulting wastewater is toxic and difficult to remove. Methods: The experimental culture was set up for the biodegradation of the azo dye AB113, and the cell growth and dye decolorization were monitored. Transcriptome sequencing was performed in the presence and absence of AB113 treatment. The key pathways and enzymes involved in AB113 degradation were found through pathway analysis and enrichment software (GO, EggNog and KEGG). Results: S. melonis B-2 achieved more than 80% decolorization within 24 h (50 and 100 mg/L dye). There was a positive relationship between cell growth and the azo dye degradation rate. The expression level of enzymes involved in benzoate and naphthalene degradation pathways (NADH quinone oxidoreductase, N-acetyltransferase and aromatic ring-hydroxylating dioxygenase) increased significantly after the treatment of AB113. Conclusions: Benzoate and naphthalene degradation pathways were the key pathways for AB113 degradation. NADH quinone oxidoreductase, N-acetyltransferase, aromatic ring-hydroxylating dioxygenase and CYP450 were the key enzymes for AB113 degradation. This study provides evidence for the process of AB113 biodegradation at the molecular and biochemical level that will be useful in monitoring the dye wastewater treatment process at the full-scale treatment.

Details

Title
Transcriptomic Analysis of Degradative Pathways for Azo Dye Acid Blue 113 in Sphingomonas melonis B-2 from the Dye Wastewater Treatment Process
Author
Aalfin-Emmanuel Santhanarajan 1   VIAFID ORCID Logo  ; Rhee, Chaeyoung 2 ; Woo, Jun Sul 3   VIAFID ORCID Logo  ; Yoo, Keunje 1   VIAFID ORCID Logo  ; Seong, Hoon Je 3   VIAFID ORCID Logo  ; Hong-Gi, Kim 4 ; Sung-Cheol Koh 1 

 Department of Environmental Engineering, Korea Maritime and Ocean University, Yeongdo-gu, Busan 49112, Korea; [email protected] (A.-E.S.); [email protected] (K.Y.) 
 Department of Energy Engineering, Future Convergence Technology Research Institute, Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea; [email protected] 
 Department of Systems Biotechnology, Chung-Ang University, Anseong 06974, Korea; [email protected] (W.J.S.); [email protected] (H.J.S.) 
 Bayo Inc., Jinju 52665, Korea; [email protected] 
First page
438
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
20762607
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2633029578
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.