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Abstract
Integrative expression Quantitative Trait Loci (eQTL) analysis found that rs8180040 was significantly associated with Coiled-coil domain containing 12 (CCDC12) in colon adenocarcinoma (COAD) patients. Immunohistochemical staining and western blotting confirmed CCDC12 was highly expressed in COAD tissues, which was consistent with RNA-Seq data from the TCGA database. Knockdown of CCDC12 could significantly reduce proliferation, migration, invasion, and tumorigenicity of colon cancer cells, while exogenous overexpression of CCDC12 had the opposite effect. Four plex Isobaric Tags for Relative and Absolute Quantitation assays were performed to determine its function and potential regulatory mechanism and demonstrated that overexpression of CCDC12 would change proteins on the adherens junction pathway. Overexpressed Snail and knocked down CCDC12 subsequently in SW480 cells, and we found that overexpression of Snail did not significantly change CCDC12 levels in SW480 cells, while knockdown of CCDC12 reduced that of Snail. CCDC12 plays a significant role in tumorigenesis, development, and invasion of COAD and may affect the epithelial to mesenchymal transformation process of colon cancer cells by regulating the Snail pathway.
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1 Shandong Provincial Hospital Affiliated to Shandong First Medical University, Department of Gastrointestinal Surgery, Jinan, China (GRID:grid.460018.b) (ISNI:0000 0004 1769 9639); Shandong University, Cheeloo College of Medicine, Jinan, China (GRID:grid.27255.37) (ISNI:0000 0004 1761 1174)
2 Shandong Provincial Hospital Affiliated to Shandong First Medical University, Department of Gastrointestinal Surgery, Jinan, China (GRID:grid.460018.b) (ISNI:0000 0004 1769 9639)
3 Shandong Provincial Hospital Affiliated to Shandong First Medical University, Personnel Office, Jinan, China (GRID:grid.460018.b) (ISNI:0000 0004 1769 9639)
4 Qilu Children’s Hospital of Shandong University, Hematology and Oncology, Jinan, China (GRID:grid.27255.37) (ISNI:0000 0004 1761 1174)
5 Shandong Provincial Hospital Affiliated to Shandong First Medical University, Department of Gastrointestinal Surgery, Jinan, China (GRID:grid.460018.b) (ISNI:0000 0004 1769 9639); Shandong University of Traditional Chinese Medicine, Center for Post-doctoral Studies, Jinan, China (GRID:grid.464402.0) (ISNI:0000 0000 9459 9325)