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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Surface engineering is a promising strategy to limit or prevent the formation of biofilms. The use of topographic cues to influence early stages of biofilm formationn has been explored, yet many fundamental questions remain unanswered. In this work, we develop a topological model supported by direct experimental evidence, which is able to explain the effect of local topography on the fate of bacterial micro-colonies of Staphylococcus spp. We demonstrate how topological memory at the single-cell level, characteristic of this genus of Gram-positive bacteria, can be exploited to influence the architecture of micro-colonies and the average number of surface anchoring points over nano-patterned surfaces, formed by vertically aligned silicon nanowire arrays that can be reliably produced on a commercial scale, providing an excellent platform to investigate the effect of topography on the early stages of Staphylococcus spp. colonisation. The surfaces are not intrinsically antimicrobial, yet they delivered a topography-based bacteriostatic effect and a significant disruption of the local morphology of micro-colonies at the surface. The insights from this work could open new avenues towards designed technologies for biofilm engineering and prevention, based on surface topography.

Details

Title
Effect of Local Topography on Cell Division of Staphylococcus spp.
Author
Sorzabal-Bellido, Ioritz 1   VIAFID ORCID Logo  ; Barbieri, Luca 2   VIAFID ORCID Logo  ; Beckett, Alison J 3   VIAFID ORCID Logo  ; Prior, Ian A 3 ; Susarrey-Arce, Arturo 4 ; Tiggelaar, Roald M 5   VIAFID ORCID Logo  ; Fothergill, Joanne 6 ; Raval, Rasmita 1 ; Diaz Fernandez, Yuri A 1   VIAFID ORCID Logo 

 Surface Science Research Centre and Open Innovation Hub for Antimicrobial Surfaces, Department of Chemistry, University of Liverpool, Liverpool L69 7ZD, UK; [email protected] (I.S.-B.); [email protected] (L.B.) 
 Surface Science Research Centre and Open Innovation Hub for Antimicrobial Surfaces, Department of Chemistry, University of Liverpool, Liverpool L69 7ZD, UK; [email protected] (I.S.-B.); [email protected] (L.B.); Institute of Infection and Global Health, University of Liverpool, Liverpool L69 3BX, UK; [email protected] 
 Biomedical Electron Microscopy Unit, University of Liverpool, Liverpool L69 3BX, UK; [email protected] (A.J.B.); [email protected] (I.A.P.) 
 Mesoscale Chemical Systems, MESA+ Institute, University of Twente, 7522 NB Enschede, The Netherlands; [email protected] 
 NanoLab Cleanroom, MESA+ Institute, University of Twente, 7522 NB Enschede, The Netherlands; [email protected] 
 Institute of Infection and Global Health, University of Liverpool, Liverpool L69 3BX, UK; [email protected] 
First page
683
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
20794991
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2633214555
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.