Full text

Turn on search term navigation

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Current study investigated effects of elevated hydrostatic pressure exposure in the presence of mild heat and natural antimicrobials against Staphylococcus aureus. Hydrostatic pressure of 350 to 550 MPa with nisin (5000 IU/mL), carvacrol, or caprylic acid (0.5% v/v) were applied for the reduction in four-strain mixture of S. aureus in HEPES buffer at 4 and 40 °C for up to 7 min. Results were statistically analyzed by ANOVA and D-values were additionally calculated using best-fitted linear model. Prior to exposure to treatments at 4 °C, counts of the pathogen were 7.95 ± 0.4 log CFU/mL and were reduced (p < 0.05) to 6.44 ± 0.3 log CFU/mL after 7 min of treatment at 450 MPa. D-value associated with this treatment was 5.34 min (R2 = 0.72). At 40 °C, counts were 8.21 ± 0.7 and 5.77 ± 0.3 log CFU/mL before and after the 7-min treatments, respectively. D-value associated with 40 °C treatment was 3.30 min (R2 = 0.62). Application of the antimicrobials provided additional pathogen reduction augmentation for treatments < 5 min. The results of the current study could be incorporated for meeting regulatory requirements such as Food Code, HACCP, and Preventive Control for Human Food of Food Safety Modernization Act for assuring microbiological safety of products against this prevalent pathogen of public health concern.

Details

Title
Sensitivity of Planktonic Cells of Staphylococcus aureus to Elevated Hydrostatic Pressure as Affected by Mild Heat, Carvacrol, Nisin, and Caprylic Acid
Author
Jyothi, George 1 ; Aras, Sadiye 1 ; Kabir, Md Niamul 2 ; Wadood, Sabrina 2 ; Chowdhury, Shahid 2 ; Aliyar Cyrus Fouladkhah 3 

 Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USA; [email protected] (J.G.); [email protected] (S.A.); [email protected] (M.N.K.); [email protected] (S.W.); [email protected] (S.C.); Department of Biological Sciences, Tennessee State University, Nashville, TN 37209, USA 
 Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USA; [email protected] (J.G.); [email protected] (S.A.); [email protected] (M.N.K.); [email protected] (S.W.); [email protected] (S.C.) 
 Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USA; [email protected] (J.G.); [email protected] (S.A.); [email protected] (M.N.K.); [email protected] (S.W.); [email protected] (S.C.); Cooperative Extension Program, Tennessee State University, Nashville, TN 37209, USA 
First page
7033
Publication year
2020
Publication date
2020
Publisher
MDPI AG
ISSN
1661-7827
e-ISSN
1660-4601
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2635380607
Copyright
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.