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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Salmonella spp. continues to figure prominently in world epidemiological registries as one of the leading causes of bacterial foodborne disease. We characterised 43 Brazilian lineages of Salmonella Typhimurium (ST) strains, characterized drug resistance patterns, tested copper (II) complex as control options, and proposed effective antimicrobial measures. The minimum inhibitory concentration was evaluated for seven antimicrobials, isolated and combined with the copper (II) complex [Cu(4-FH)(phen)(ClO4)2] (4-FH = 4-fluorophenoxyacetic acid hydrazide and phen = 1,10-phenanthroline), known as DRI-12, in planktonic and sessile ST. In parallel, 42 resistance genes were screened (PCR/microarray). All strains were multidrug resistant (MDR). Resistance to carbapenems and polymyxins (86 and 88%, respectively) have drawn attention to the emergence of the problem in Brazil, and resistance is observed also to CIP and CFT (42 and 67%, respectively), the drugs of choice in treatment. Resistance to beta-lactams was associated with the genes blaTEM/blaCTX-M in 39% of the strains. Lower concentrations of DRI-12 (62.7 mg/L, or 100 μM) controlled planktonic and sessile ST in relation to AMP/SUL/TET and AMP/SUL/TET/COL, respectively. The synergistic effect provided by DRI-12 was significant for COL/CFT and COL/AMP in planktonic and sessile ST, respectively, and represents promising alternatives for the control of MDR ST.

Details

Title
A Ternary Copper (II) Complex with 4-Fluorophenoxyacetic Acid Hydrazide in Combination with Antibiotics Exhibits Positive Synergistic Effect against Salmonella Typhimurium
Author
Guilherme Paz Monteiro 1 ; Torres de Melo, Roberta 1   VIAFID ORCID Logo  ; Guidotti-Takeuchi, Micaela 1   VIAFID ORCID Logo  ; Carolyne Ferreira Dumont 1   VIAFID ORCID Logo  ; Rosanne Aparecida Capanema Ribeiro 1 ; Guerra, Wendell 2 ; Luana Munique Sousa Ramos 2 ; Drielly Aparecida Paixão 2   VIAFID ORCID Logo  ; Fernanda Aparecida Longato dos Santos 1 ; Dália dos Prazeres Rodrigues 3 ; Boleij, Peter 4 ; Hoepers, Patrícia Giovana 5 ; Daise Aparecida Rossi 1 

 Laboratory of Molecular Epidemiology, Federal University of Uberlândia, Uberlândia 38402-018, Brazil; [email protected] (G.P.M.); [email protected] (M.G.-T.); [email protected] (C.F.D.); [email protected] (R.A.C.R.); [email protected] (F.A.L.d.S.); [email protected] (D.A.R.) 
 Institute of Chemistry, Federal University of Uberlândia, Uberlândia 38402-018, Brazil; [email protected] (W.G.); [email protected] (L.M.S.R.); [email protected] (D.A.P.) 
 Laboratory of Enterobacteriaceae, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil; [email protected] 
 Check-Points B.V., 6709 PD Wageningen, The Netherlands; [email protected] 
 Postgraduate Program in Veterinary Science, Federal University of Uberlândia, Uberlândia 38402-018, Brazil; [email protected] 
First page
388
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
20796382
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2642335547
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.