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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Keratin biomaterials with high molecular weights were intensively investigated but few are marketed due to complex methods of extraction and preparation and limited understanding of their influence on cells behavior. In this context the aim of this research was to elucidate decisive molecular factors for skin homeostasis restoration induced by two low molecular weight keratin hydrolysates extracted and conditioned through a simple and green method. Two keratin hydrolysates with molecular weights of 3758 and 12,400 Da were physico-chemically characterized and their structure was assessed by circular dichroism (CD) and FTIR spectroscopy in view of bioactive potential identification. Other investigations were focused on several molecular factors: α1, α2 and β1 integrin mediated signals, cell cycle progression in pro-inflammatory conditions (TNFα/LPS stimulated keratinocytes and fibroblasts) and ICAM-1/VCAM-1 inhibition in human vascular endothelial cells. Flow cytometry techniques demonstrated a distinctive pattern of efficacy: keratin hydrolysates over-expressed α1 and α2 subunits, responsible for tight bounds between fibroblasts and collagen or laminin 1; both actives stimulated the epidermal turn-over and inhibited VCAM over-expression in pro-inflammatory conditions associated with bacterial infections. Our results offer mechanistic insights in wound healing signaling factors modulated by the two low molecular weight keratin hydrolysates which still preserve bioactive secondary structure.

Details

Title
Bioactive Low Molecular Weight Keratin Hydrolysates for Improving Skin Wound Healing
Author
Olariu, Laura 1 ; Brindusa, Georgiana Dumitriu 2 ; Gaidau, Carmen 3   VIAFID ORCID Logo  ; Stanca, Maria 3 ; Tanase, Luiza Mariana 2   VIAFID ORCID Logo  ; Ene, Manuela Diana 2   VIAFID ORCID Logo  ; Stanculescu, Ioana-Rodica 4   VIAFID ORCID Logo  ; Tablet, Cristina 5   VIAFID ORCID Logo 

 SC Biotehnos SA, 3–5 Gorunului Street, 075100 Otopeni, Romania; [email protected] (L.O.); [email protected] (B.G.D.); [email protected] (L.M.T.); [email protected] (M.D.E.); Academy of Romanian Scientists, 3 Ilfov Street, 030167 Bucharest, Romania 
 SC Biotehnos SA, 3–5 Gorunului Street, 075100 Otopeni, Romania; [email protected] (L.O.); [email protected] (B.G.D.); [email protected] (L.M.T.); [email protected] (M.D.E.) 
 Leather Research Department, National Institute for Textiles and Leather Division Leather and Footwear Research Institute (ICPI), 93 Ion Minulescu Street, 031215 Bucharest, Romania; [email protected] 
 Horia Hulubei National Institute of Research and Development for Physics and Nuclear Engineering, 30 Reactorului Str., 077125 Magurele, Romania; [email protected]; Department of Physical Chemistry, University of Bucharest, 4–12 Regina Elisabeta Bd., 030018 Bucharest, Romania; [email protected] 
 Department of Physical Chemistry, University of Bucharest, 4–12 Regina Elisabeta Bd., 030018 Bucharest, Romania; [email protected]; Faculty of Pharmacy, Titu Maiorescu University, 16 Gh. Sincai Bd., 040317 Bucharest, Romania 
First page
1125
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
20734360
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2642617658
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.