Abstract

Investigation of the complex relationships and dependencies of multiple cellular processes that govern cardiac physiology and pathophysiology requires simultaneous dynamic assessment of multiple parameters. In this study, we introduce triple-parametric optical mapping to simultaneously image metabolism, electrical excitation, and calcium signaling from the same field of view and demonstrate its application in the field of drug testing and cardiovascular research. We applied this metabolism-excitation-contraction coupling (MECC) methodology to test the effects of blebbistatin, 4-aminopyridine and verapamil on cardiac physiology. While blebbistatin and 4-aminopyridine alter multiple aspects of cardiac function suggesting off-target effects, the effects of verapamil were on-target and it altered only one of ten tested parameters. Triple-parametric optical mapping was also applied during ischemia and reperfusion; and we identified that metabolic changes precede the effects of ischemia on cardiac electrophysiology.

Simultaneous triple-parametric optical mapping of transmembrane potential, intracellular calcium, and metabolic markers for cardiac physiology shows that metabolic changes precede the effects of ischemia on cardiac electrophysiology.

Details

Title
Simultaneous triple-parametric optical mapping of transmembrane potential, intracellular calcium and NADH for cardiac physiology assessment
Author
George, Sharon A 1   VIAFID ORCID Logo  ; Lin Zexu 2 ; Efimov, Igor R 1   VIAFID ORCID Logo 

1  The George Washington University, Department of Biomedical Engineering, Washington, USA (GRID:grid.253615.6) (ISNI:0000 0004 1936 9510); Northwestern University, Department of Biomedical Engineering, Chicago, USA (GRID:grid.16753.36) (ISNI:0000 0001 2299 3507) 
2  The George Washington University, Department of Biomedical Engineering, Washington, USA (GRID:grid.253615.6) (ISNI:0000 0004 1936 9510) 
Publication year
2022
Publication date
2022
Publisher
Nature Publishing Group
e-ISSN
23993642
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s42003-022-03279-y
ProQuest document ID
2647482575
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.