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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Several Prunus species are among the most important cultivated stone fruits in the Mediterranean region, and there is an urgent need to obtain rootstocks with specific adaptations to challenging environmental conditions. The development of adventitious roots (ARs) is an evolutionary mechanism of high relevance for stress tolerance, which has led to the development of environmentally resilient plants. As a first step towards understanding the genetic determinants involved in AR formation in Prunus sp., we evaluated the rooting of hardwood cuttings from five Prunus rootstocks (Adafuel, Adarcias, Cadaman, Garnem, and GF 677) grown in hydroponics. We found that auxin-induced callus and rooting responses were strongly genotype-dependent. To investigate the molecular mechanisms involved in these differential responses, we performed a time-series study of AR formation in two rootstocks with contrasting rooting performance, Garnem and GF 677, by culturing in vitro microcuttings with and without auxin treatment (0.9 mg/L of indole-3-butyric acid [IBA]). Despite showing a similar histological structure, Garnem and GF677 rootstocks displayed dynamic changes in endogenous hormone homeostasis involving metabolites such as indole-3-acetic acid (IAA) conjugated to aspartic acid (IAA-Asp), and these changes could explain the differences observed during rooting.

Details

Title
Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks
Author
Justamante, María Salud 1 ; Mhimdi, Mariem 1 ; Molina-Pérez, Marta 1   VIAFID ORCID Logo  ; Albacete, Alfonso 2   VIAFID ORCID Logo  ; María Ángeles Moreno 3   VIAFID ORCID Logo  ; Mataix, Inés 4 ; Pérez-Pérez, José Manuel 1   VIAFID ORCID Logo 

 Instituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain; [email protected] (M.S.J.); [email protected] (M.M.); [email protected] (M.M.-P.) 
 Departmento de Nutrición Vegetal, CEBAS-CSIC, 30100 Murcia, Spain; [email protected] 
 Department of Pomology, Estación Experimental de Aula Dei-CSIC, 50059 Zaragoza, Spain; [email protected] 
 Invisa Biotecnología Vegetal S.L., 30410 Caravaca de la Cruz, Spain; [email protected] 
First page
913
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
22237747
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2649019806
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.