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© 2019. This work is published under https://creativecommons.org/licenses/by/4.0/legalcode (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Background:

AIDS-associated Opportunistic Infections (OIs) have significant morbidity and mortality and can be diagnostically challenging, requiring invasive procedures as well as a combination of culture and targeted molecular approaches.

Objective:

We aimed to demonstrate the clinical utility of Next-generation Sequencing (NGS) in pathogen identification; NGS is a maturing technology enabling the detection of miniscule amounts of cell-free microbial DNA from the bloodstream.

Methods:

We utilized a novel Next-generation Sequencing (NGS) test on plasma samples to diagnose a series of HIV-associated OIs that were diagnostically confirmed through conventional microbial testing.

Results:

In all cases, NGS test results were available sooner than conventional testing. This is the first case series demonstrating the utility of whole-genome NGS testing to identify OIs from plasma in HIV/AIDS patients.

Conclusion:

NGS approaches present a clinically-actionable, comprehensive means of diagnosing OIs and other systemic infections while avoiding the labor, expense, and delays of multiple tests and invasive procedures.

Details

Title
Utility of Whole-Genome Next-Generation Sequencing of Plasma in Identifying Opportunistic Infections in HIV/AIDS
Author
Zhou, Yang; Hemmige, Vagish; Dalai, Sudeb C; Hong, David K; Muldrew, Kenneth; Mayar Al Mohajer
Pages
7-11
Section
CASE REPORT
Publication year
2019
Publication date
2019
Publisher
Benham Science Publishers
ISSN
18746136
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2669553181
Copyright
© 2019. This work is published under https://creativecommons.org/licenses/by/4.0/legalcode (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.