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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Radiotherapy (RT) with accelerated beams of charged particles (protons and carbon ions), also known as hadrontherapy, is a treatment modality that is increasingly being adopted thanks to the several benefits that it grants compared to conventional radiotherapy (CRT) treatments performed by means of high-energy photons/electrons. Hence, information about the biomolecular effects in exposed cells caused by such particles is needed to better realize the underlying radiobiological mechanisms and to improve this therapeutic strategy. To this end, Fourier transform infrared microspectroscopy (μ-FT-IR) can be usefully employed, in addition to long-established radiobiological techniques, since it is currently considered a helpful tool for examining radiation-induced cellular changes. In the present study, MCF-10A breast cells were chosen to evaluate the effects of proton exposure using μ-FT-IR. They were exposed to different proton doses and fixed at various times after exposure to evaluate direct effects due to proton exposure and the kinetics of DNA damage repair. Irradiated and control cells were examined in transflection mode using low-e substrates that have been recently demonstrated to offer a fast and direct way to examine proton-exposed cells. The acquired spectra were analyzed using a deconvolution procedure and a ratiometric approach, both of which showed the different contributions of DNA, protein, lipid, and carbohydrate cell components. These changes were particularly significant for cells fixed 48 and 72 h after exposure. Lipid changes were related to variations in membrane fluidity, and evidence of DNA damage was highlighted. The analysis of the Amide III band also indicated changes that could be related to different enzyme contributions in DNA repair.

Details

Title
Evaluation of Proton-Induced Biomolecular Changes in MCF-10A Breast Cells by Means of FT-IR Microspectroscopy
Author
Ricciardi, Valerio 1   VIAFID ORCID Logo  ; Portaccio, Marianna 2   VIAFID ORCID Logo  ; Lasalvia, Maria 3   VIAFID ORCID Logo  ; Cammarata, Francesco Paolo 4   VIAFID ORCID Logo  ; Pisciotta, Pietro 5   VIAFID ORCID Logo  ; Perna, Giuseppe 3   VIAFID ORCID Logo  ; Capozzi, Vito 3   VIAFID ORCID Logo  ; Petringa, Giada 6   VIAFID ORCID Logo  ; Manti, Lorenzo 7   VIAFID ORCID Logo  ; Lepore, Maria 2   VIAFID ORCID Logo 

 Istituto Nazionale di Fisica Nucleare—Sezione di Napoli, 80100 Napoli, Italy; [email protected] (V.R.); [email protected] (L.M.) 
 Dipartimento di Medicina Sperimentale, Università della Campania “Luigi Vanvitelli”, 80138 Napoli, Italy; [email protected] (M.P.); [email protected] (M.L.) 
 Dipartimento di Medicina Clinica e Sperimentale, Università di Foggia, 71122 Foggia, Italy; [email protected] (M.L.); [email protected] (G.P.) 
 Istituto di Bioimmagini e Fisiologia Molecolare—Consiglio Nazionale delle Ricerche, 90015 Cefalù, Italy; [email protected]; Istituto Nazionale di Fisica Nucleare—Laboratori Nazionali del Sud, 95123 Catania, Italy; [email protected] (P.P.); [email protected] (G.P.) 
 Istituto Nazionale di Fisica Nucleare—Laboratori Nazionali del Sud, 95123 Catania, Italy; [email protected] (P.P.); [email protected] (G.P.); Dipartimento di Fisica ed Astronomia “E. Majorana”, Università degli Studi di Catania, 95123 Catania, Italy; University Medical Center Groningen, Department of Radiotherapy, University of Groningen, 9713 GZ Groningen, The Netherlands 
 Istituto Nazionale di Fisica Nucleare—Laboratori Nazionali del Sud, 95123 Catania, Italy; [email protected] (P.P.); [email protected] (G.P.) 
 Istituto Nazionale di Fisica Nucleare—Sezione di Napoli, 80100 Napoli, Italy; [email protected] (V.R.); [email protected] (L.M.); Dipartimento di Fisica “E. Pancini”, Università degli Studi di Napoli “Federico II”, 80126 Napoli, Italy 
First page
5074
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
20763417
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2670082013
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.