It appears you don't have support to open PDFs in this web browser. To view this file, Open with your PDF reader
Abstract
Intestinal organoids are advanced cellular models, which are widely used in mammalian studies to mimic and study in vivo intestinal function and host–pathogen interactions. Growth factors WNT3 and RSPO1 are crucial for the growth of intestinal organoids. Chicken intestinal organoids are currently cultured with mammalian Wnt3a and Rspo1, however, maintaining their longevity has shown to be challenging. Based on the limited homology between mammalian and avian RSPO1, we expect that chicken-derived factors are required for the organoid cultures. Isolated crypts from embryonic tissue of laying hens were growing in the presence of chicken WNT3 and RSPO1, whereas growth in the presence of mammalian Wnt3a and Rspo1 was limited. Moreover, the growth was increased by using Prostaglandin E2 (PGE2) and a Forkhead box O1-inhibitor (FOXO1-inhibitor), allowing to culture these organoids for 15 passages. Furthermore, stem cells maintained their ability to differentiate into goblets, enterocytes and enteroendocrine cells in 2D structures. Overall, we show that chicken intestinal organoids can be cultured for multiple passages using chicken-derived WNT3 and RSPO1, PGE2, and FOXO1-inhibitor.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details
1 Maastricht University, Centre for Healthy Eating and Food Innovation, Faculty of Science and Engineering, Venlo, The Netherlands (GRID:grid.5012.6) (ISNI:0000 0001 0481 6099)
2 Utrecht University, Division Infectious Diseases and Immunology, Department Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht, The Netherlands (GRID:grid.5477.1) (ISNI:0000000120346234)
3 University of Applied Sciences Utrecht, Innovative Testing in Life Sciences and Chemistry, Research Centre Healthy and Sustainable Living, Utrecht, The Netherlands (GRID:grid.438049.2) (ISNI:0000 0001 0824 9343)
4 Utrecht University, Division Farm Animal Health, Department Population Health Sciences, Faculty of Veterinary Medicine, Utrecht, The Netherlands (GRID:grid.5477.1) (ISNI:0000000120346234)
5 Utrecht University, Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht, The Netherlands (GRID:grid.5477.1) (ISNI:0000000120346234)
6 Utrecht University, Division Infectious Diseases and Immunology, Department Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht, The Netherlands (GRID:grid.5477.1) (ISNI:0000000120346234); Wageningen University & Research, Department of Animal Sciences, Cell Biology and Immunology Group, Wageningen, The Netherlands (GRID:grid.4818.5) (ISNI:0000 0001 0791 5666)
7 University of Applied Sciences Utrecht, Innovative Testing in Life Sciences and Chemistry, Research Centre Healthy and Sustainable Living, Utrecht, The Netherlands (GRID:grid.438049.2) (ISNI:0000 0001 0824 9343); Utrecht University, Department of Population Health Sciences, Institute for Risk Assessment Sciences, Utrecht, The Netherlands (GRID:grid.5477.1) (ISNI:0000000120346234)