Abstract

A growing number of children born are conceived through in vitro fertilisation (IVF), which has been linked to an increased risk of adverse perinatal outcomes, as well as altered growth profiles and cardiometabolic differences in the resultant individuals. Some of these outcomes have also been shown to be influenced by the use of different IVF culture media and this effect is hypothesised to be mediated epigenetically, e.g. through the methylome. As such, we profiled the umbilical cord blood methylome of IVF neonates that underwent preimplantation embryo development in two different IVF culture media (G5 or HTF), using the Infinium Human Methylation EPIC BeadChip. We found no significant methylation differences between the two groups in terms of: (i) systematic differences at CpG sites or regions, (ii) imprinted sites/genes or birth weight-associated sites, (iii) stochastic differences presenting as DNA methylation outliers or differentially variable sites, and (iv) epigenetic gestational age acceleration.

Details

Title
Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences
Author
Koeck, Rebekka M. 1   VIAFID ORCID Logo  ; Busato, Florence 2 ; Tost, Jorg 2 ; Consten, Dimitri 3 ; van Echten-Arends, Jannie 4 ; Mastenbroek, Sebastiaan 5   VIAFID ORCID Logo  ; Wurth, Yvonne 3 ; Remy, Sylvie 6   VIAFID ORCID Logo  ; Langie, Sabine 7 ; Nawrot, Tim S. 8 ; Plusquin, Michelle 9 ; Alfano, Rossella 9   VIAFID ORCID Logo  ; Bijnens, Esmée M. 10 ; Gielen, Marij 11 ; van Golde, Ron 12 ; Dumoulin, John C. M. 12 ; Brunner, Han 13 ; van Montfoort, Aafke P. A. 12 ; Zamani Esteki, Masoud 1 

1  Maastricht University Medical Centre+, Department of Clinical Genetics, Maastricht, The Netherlands (GRID:grid.412966.e) (ISNI:0000 0004 0480 1382); Maastricht University, Department of Genetics and Cell Biology, GROW School for Oncology and Reproduction, Maastricht, The Netherlands (GRID:grid.5012.6) (ISNI:0000 0001 0481 6099) 
2  CEA – institut de Biologie François Jacob, Université Paris Saclay, Laboratory for Epigenetics & Environment, Centre National de Recherche en Genomique Humaine, Evry, France (GRID:grid.460789.4) (ISNI:0000 0004 4910 6535) 
3  St. Elisabeth-TweeSteden Hospital, Center for Reproductive Medicine, Tilburg, the Netherlands (GRID:grid.416373.4) (ISNI:0000 0004 0472 8381) 
4  University of Groningen, Section of Reproductive Medicine, Department of Obstetrics and Gynecology, University Medical Center Groningen, Groningen, the Netherlands (GRID:grid.4830.f) (ISNI:0000 0004 0407 1981) 
5  University of Amsterdam, Center for Reproductive Medicine, Amsterdam Reproduction & Development Research Institute, Amsterdam UMC, Amsterdam, the Netherlands (GRID:grid.7177.6) (ISNI:0000000084992262) 
6  Health Unit, Flemish Institute for Technological Research (VITO), Mol, Belgium (GRID:grid.6717.7) (ISNI:0000000120341548) 
7  Health Unit, Flemish Institute for Technological Research (VITO), Mol, Belgium (GRID:grid.6717.7) (ISNI:0000000120341548); Maastricht University, Department of Pharmacology & Toxicology, School for Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht, The Netherlands (GRID:grid.5012.6) (ISNI:0000 0001 0481 6099) 
8  Hasselt University, Centre for Environmental Sciences, Diepenbeek, Belgium (GRID:grid.12155.32) (ISNI:0000 0001 0604 5662); Leuven University (KU Leuven), Department of Public Health and Primary Care, Leuven, Belgium (GRID:grid.5596.f) (ISNI:0000 0001 0668 7884) 
9  Hasselt University, Centre for Environmental Sciences, Diepenbeek, Belgium (GRID:grid.12155.32) (ISNI:0000 0001 0604 5662) 
10  Hasselt University, Centre for Environmental Sciences, Diepenbeek, Belgium (GRID:grid.12155.32) (ISNI:0000 0001 0604 5662); Ghent University Hospital, Department of Human Structure and Repair, Ghent, Belgium (GRID:grid.410566.0) (ISNI:0000 0004 0626 3303) 
11  Maastricht University Medical Centre, Department of Epidemiology and Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht, the Netherlands (GRID:grid.412966.e) (ISNI:0000 0004 0480 1382) 
12  Maastricht University Medical Center+, Department of Obstetrics and Gynaecology, GROW School for Oncology and Reproduction, Maastricht, The Netherlands (GRID:grid.412966.e) (ISNI:0000 0004 0480 1382) 
13  Maastricht University Medical Centre+, Department of Clinical Genetics, Maastricht, The Netherlands (GRID:grid.412966.e) (ISNI:0000 0004 0480 1382); Radboud University Medical Center, Department of Human Genetics, Nijmegen, The Netherlands (GRID:grid.10417.33) (ISNI:0000 0004 0444 9382) 
Publication year
2022
Publication date
2022
Publisher
Nature Publishing Group
e-ISSN
20567944
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41525-022-00310-3
ProQuest document ID
2682015835
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.