Abstract

Cassava mosaic disease (CMD) suppresses cassava yields across the tropics. The dominant CMD2 locus confers resistance to cassava mosaic geminiviruses. It has been reported that CMD2-type landraces lose resistance after regeneration through de novo morphogenesis. As full genome bisulfite sequencing failed to uncover an epigenetic mechanism for this loss of resistance, whole genome sequencing and genetic variant analysis was performed and the CMD2 locus was fine-mapped to a 190 kilobase interval. Collectively, these data indicate that CMD2-type resistance is caused by a nonsynonymous, single nucleotide polymorphism in DNA polymerase δ subunit 1 (MePOLD1) located within this region. Virus-induced gene silencing of MePOLD1 in a CMD-susceptible cassava variety produced a recovery phenotype typical of CMD2-type resistance. Analysis of other CMD2-type cassava varieties identified additional candidate resistance alleles within MePOLD1. Genetic variation of MePOLD1, therefore, could represent an important genetic resource for resistance breeding and/or genome editing, and elucidating mechanisms of resistance to geminiviruses.

Cassava mosaic disease is caused by geminiviruses and suppresses cassava yields throughout the tropics. Here, the authors show that mutations in MePOLD1, encoding DNA polymerase δ subunit 1, co-segregate with CMD2, the major source of genetic resistance for this disease.

Details

Title
Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses
Author
Lim, Yi-Wen 1   VIAFID ORCID Logo  ; Mansfeld, Ben N. 2 ; Schläpfer, Pascal 1   VIAFID ORCID Logo  ; Gilbert, Kerrigan B. 2   VIAFID ORCID Logo  ; Narayanan, Narayanan N. 2 ; Qi, Weihong 3 ; Wang, Qi 2 ; Zhong, Zhenhui 4   VIAFID ORCID Logo  ; Boyher, Adam 2 ; Gehan, Jackson 2 ; Beyene, Getu 2   VIAFID ORCID Logo  ; Lin, Zuh-Jyh Daniel 2 ; Esuma, Williams 5 ; Feng, Suhua 4 ; Chanez, Christelle 1   VIAFID ORCID Logo  ; Eggenberger, Nadine 1   VIAFID ORCID Logo  ; Adiga, Gerald 5 ; Alicai, Titus 5   VIAFID ORCID Logo  ; Jacobsen, Steven E. 6   VIAFID ORCID Logo  ; Taylor, Nigel J. 2 ; Gruissem, Wilhelm 7   VIAFID ORCID Logo  ; Bart, Rebecca S. 2   VIAFID ORCID Logo 

 ETH Zürich, Institute of Molecular Plant Biology, Department of Biology, Zürich, Switzerland (GRID:grid.5801.c) (ISNI:0000 0001 2156 2780) 
 Donald Danforth Plant Science Center, St. Louis, USA (GRID:grid.34424.35) (ISNI:0000 0004 0466 6352) 
 ETH Zurich and University of Zurich, Functional Genomics Center Zurich, Zurich, Switzerland (GRID:grid.7400.3) (ISNI:0000 0004 1937 0650) 
 University of California Los Angeles, Department of Molecular, Cell and Developmental Biology, Los Angeles, USA (GRID:grid.19006.3e) (ISNI:0000 0000 9632 6718) 
 National Crops Resources Research Institute, Root Crops Program, Kampala, Uganda (GRID:grid.463519.c) (ISNI:0000 0000 9021 5435) 
 University of California Los Angeles, Department of Molecular, Cell and Developmental Biology, Los Angeles, USA (GRID:grid.19006.3e) (ISNI:0000 0000 9632 6718); Howard Hughes Medical Institute University of California Los Angeles, Los Angeles, USA (GRID:grid.19006.3e) (ISNI:0000 0000 9632 6718) 
 ETH Zürich, Institute of Molecular Plant Biology, Department of Biology, Zürich, Switzerland (GRID:grid.5801.c) (ISNI:0000 0001 2156 2780); National Chung Hsing University, Biotechnology Center, Taichung City, Taiwan (GRID:grid.5801.c) 
Publication year
2022
Publication date
2022
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2685814651
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.