It appears you don't have support to open PDFs in this web browser. To view this file, Open with your PDF reader
Abstract
We investigated gluteal (GSAT) and abdominal subcutaneous adipose tissue (ASAT) DNA methylation of FKBP5 in response to a 12-week intervention in African women with obesity, as well as the effect of the rs1360780 single nucleotide polymorphism (SNP) on FKBP5 methylation, gene expression and post-exercise training adaptations in obesity and metabolic related parameters. Exercise (n = 19) participants underwent 12-weeks of supervised aerobic and resistance training while controls (n = 12) continued their usual behaviours. FKBP5 methylation was measured in GSAT and ASAT using pyrosequencing. SNP and gene expression analyses were conducted using quantitative real-time PCR. Exercise training induced FKBP5 hypermethylation at two CpG dinucleotides within intron 7. When stratified based on the rs1360780 SNP, participants with the CT genotype displayed FKBP5 hypermethylation in GSAT (p < 0.05), and ASAT displayed in both CC and CT carriers. CC allele carriers displayed improved cardiorespiratory fitness, insulin sensitivity, gynoid fat mass, and waist circumference (p < 0.05) in response to exercise training, and these parameters were attenuated in women with the CT genotype. These findings provide a basis for future studies in larger cohorts, which should assess whether FKBP5 methylation and/or genetic variants such as the rs1360780 SNP could have a significant impact on responsiveness to exercise interventions.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details
1 South African Medical Research Council, Biomedical Research and Innovation Platform, Tygerberg, South Africa (GRID:grid.415021.3) (ISNI:0000 0000 9155 0024); University of Stellenbosch, Division of Medical Physiology, Centre for Cardiometabolic Research in Africa (CARMA), Faculty of Medicine and Health Sciences, Tygerberg, South Africa (GRID:grid.11956.3a) (ISNI:0000 0001 2214 904X)
2 South African Medical Research Council, Biomedical Research and Innovation Platform, Tygerberg, South Africa (GRID:grid.415021.3) (ISNI:0000 0000 9155 0024)
3 University of the Witwatersrand, South African Medical Research Council/WITS Developmental Pathways for Health Research Unit (DPHRU), Department of Paediatrics, School of Clinical Medicine, Faculty of Health Sciences, Johannesburg, South Africa (GRID:grid.11951.3d) (ISNI:0000 0004 1937 1135); University of Cape Town, Division of Physiological Sciences, Health Through Physical Activity, Lifestyle and Sport Research Centre (HPALS), Department of Human Biology, Faculty of Health Sciences, FIMS International Collaborating Centre of Sports Medicine, Cape Town, South Africa (GRID:grid.7836.a) (ISNI:0000 0004 1937 1151)
4 University of Cape Town, Division of Physiological Sciences, Health Through Physical Activity, Lifestyle and Sport Research Centre (HPALS), Department of Human Biology, Faculty of Health Sciences, FIMS International Collaborating Centre of Sports Medicine, Cape Town, South Africa (GRID:grid.7836.a) (ISNI:0000 0004 1937 1151); South African Medical Research Council, Non-Communicable Diseases Research Unit, Tygerberg, 7505, Cape Town, South Africa (GRID:grid.415021.3) (ISNI:0000 0000 9155 0024)
5 South African Medical Research Council, Biomedical Research and Innovation Platform, Tygerberg, South Africa (GRID:grid.415021.3) (ISNI:0000 0000 9155 0024); University of Stellenbosch, Division of Medical Physiology, Centre for Cardiometabolic Research in Africa (CARMA), Faculty of Medicine and Health Sciences, Tygerberg, South Africa (GRID:grid.11956.3a) (ISNI:0000 0001 2214 904X); University of Pretoria, Department of Obstetrics and Gynaecology, Faculty of Health Sciences, Pretoria, South Africa (GRID:grid.49697.35) (ISNI:0000 0001 2107 2298)