Abstract

Section Highlights

Cas9 RNA functions as a miRNA sponge.

Let-7 is the dominant regulated miRNA by Cas9 RNA.

RNA sequence optimization of Cas9 by synonymous mutation improves its safety.

Details

Title
Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge
Author
Jiang, Junfeng  VIAFID ORCID Logo  ; Zeng, Tao; Zhang, Li; Fan, Xingfei; Jin, Qishu; Ni, Haitao; Ye, Yusheng; Cheng, Lipeng; Li, Li; Wang, Liujun; Xu, Sha; Yang, Yu; Gu, Juan; Guo, Bing; Wang, Lei; Li, Xin; Qin, Yingyi; Li, Jiaxi; Wang, Jinjiang; Chen, Xi; Wu, Minjuan; Qi-long, Ying; Qin, Xingjun; Wang, Yefei; Wang, Yue
Pages
1-8
Section
Letter to the Editor
Publication year
2022
Publication date
2022
Publisher
BioMed Central
e-ISSN
14764598
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s12943-022-01604-x
ProQuest document ID
2691577266
Copyright
© 2022. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.