Abstract

This study investigated the therapeutic effects of dry-preserved multi-layered fibroblast cell sheets (dry sheets) on cutaneous ulcers. Dry sheets were prepared by air-drying multi-layered fibroblast cell sheets (living sheets) to cease their life activities. Before in vivo application, we tested the release of growth factors into the medium to examine the mechanisms of dry sheets in wound healing. Vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) were released from both dry and living sheets, while high levels of fibroblast growth factor-2 (FGF-2) and high mobility group box 1 (HMGB1) protein were only from dry sheets. An in vitro fibroblast proliferation assay revealed that the dry sheet eluate significantly enhanced cell proliferation and VEGF and HGF production compared with living sheet eluate. FGF-2-neutralizing antibodies significantly blocked this proliferative response. In wounds created on diabetic mice, the dry sheet-treatment groups using autologous or allogeneic cells showed significantly accelerated wound closure compared with that in the no-treatment group. The storage stability of the dry sheet was better at refrigeration temperature than at room temperature and remained stable for at least 4 weeks. Our data indicated that allogeneic dry sheets represent a promising new tool for regenerative medicine that promotes wound healing.

Details

Title
Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing
Author
Matsuno, Yutaro 1 ; Yanagihara, Masashi 1 ; Ueno, Koji 1 ; Saito, Toshiro 1 ; Kurazumi, Hiroshi 1 ; Suzuki, Ryo 1 ; Katsura, Shunsaku 1 ; Oga, Atsunori 2 ; Hamano, Kimikazu 1 

 Yamaguchi University Graduate School of Medicine, Department of Surgery and Clinical Science, Ube, Japan (GRID:grid.268397.1) (ISNI:0000 0001 0660 7960) 
 Yamaguchi University Graduate School of Medicine, Department of Molecular Pathology, Ube, Japan (GRID:grid.268397.1) (ISNI:0000 0001 0660 7960) 
Publication year
2022
Publication date
2022
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2692913089
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.