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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Leptin is involved in the modulation of insulin signaling in peripheral tissues, being closely associated with changes in lipid metabolism. This adipokine modifies inflammatory pathways that can interact with insulin targets in peripheral organs; however, the mechanisms remain unclear. Inflammatory and insulin signaling targets, cytokines, adiponectin, irisin and non-esterified fatty acid (NEFA) levels and enzymes of fatty acid anabolism were studied in the gastrocnemius of chronic centrally infused leptin (L), pair-fed and control rats. The phosphorylation of signal transducer and activator of transcription 3 (STAT3) and c-Jun N-terminal kinase (JNK) was reduced in L rats (59% and 58%, respectively). The phosphorylation of the insulin receptor and Akt and adiponectin and irisin content was increased in L rats (154%, 157%, 308% and 329%, respectively). The levels of glucose-6-phosphate dehydrogenase, the mRNA content of acetyl Co-A carboxylase and NEFA concentrations were diminished in the muscles of L rats (59%, 50% and 61%, respectively). The activation of JNK correlated positively with STAT3 phosphorylation, tumoral necrosis factor-α and NEFA and negatively with irisin and Akt phosphorylation. These data suggest that the activation of insulin signaling targets and a decrease in NEFA content are associated with a reduction in muscle inflammation parameters, suggesting that leptin may integrate these pathways.

Details

Title
Chronic Central Leptin Infusion Promotes an Anti-Inflammatory Cytokine Profile Related to the Activation of Insulin Signaling in the Gastrocnemius of Male Rats
Author
Barrios, Vicente 1   VIAFID ORCID Logo  ; Guerra-Cantera, Santiago 2   VIAFID ORCID Logo  ; Martín-Rivada, Álvaro 3   VIAFID ORCID Logo  ; Canelles, Sandra 1 ; Campillo-Calatayud, Ana 4 ; Arilla-Ferreiro, Eduardo 5   VIAFID ORCID Logo  ; Frago, Laura M 2   VIAFID ORCID Logo  ; Chowen, Julie A 6   VIAFID ORCID Logo  ; Argente, Jesús 7   VIAFID ORCID Logo 

 Department of Endocrinology, Hospital Infantil Universitario Niño Jesús, Instituto de Investigación La Princesa, E-28009 Madrid, Spain; [email protected] (S.G.-C.); [email protected] (Á.M.-R.); [email protected] (S.C.); [email protected] (A.C.-C.); [email protected] (L.M.F.); [email protected] (J.A.C.); Network Center for Biomedical Research on Obesity and Nutrition, Instituto de Salud Carlos III (CIBEROBN, ISCIII), E-28009 Madrid, Spain 
 Department of Endocrinology, Hospital Infantil Universitario Niño Jesús, Instituto de Investigación La Princesa, E-28009 Madrid, Spain; [email protected] (S.G.-C.); [email protected] (Á.M.-R.); [email protected] (S.C.); [email protected] (A.C.-C.); [email protected] (L.M.F.); [email protected] (J.A.C.); Network Center for Biomedical Research on Obesity and Nutrition, Instituto de Salud Carlos III (CIBEROBN, ISCIII), E-28009 Madrid, Spain; Department of Pediatrics, Faculty of Medicine, Universidad Autónoma de Madrid, E-28029 Madrid, Spain 
 Department of Endocrinology, Hospital Infantil Universitario Niño Jesús, Instituto de Investigación La Princesa, E-28009 Madrid, Spain; [email protected] (S.G.-C.); [email protected] (Á.M.-R.); [email protected] (S.C.); [email protected] (A.C.-C.); [email protected] (L.M.F.); [email protected] (J.A.C.); Department of Pediatrics, Faculty of Medicine, Universidad Autónoma de Madrid, E-28029 Madrid, Spain 
 Department of Endocrinology, Hospital Infantil Universitario Niño Jesús, Instituto de Investigación La Princesa, E-28009 Madrid, Spain; [email protected] (S.G.-C.); [email protected] (Á.M.-R.); [email protected] (S.C.); [email protected] (A.C.-C.); [email protected] (L.M.F.); [email protected] (J.A.C.) 
 Biochemical Unit, Department of Biological Systems, Faculty of Medicine, Universidad de Alcalá, E-28871 Alcalá de Henares, Spain; [email protected] 
 Department of Endocrinology, Hospital Infantil Universitario Niño Jesús, Instituto de Investigación La Princesa, E-28009 Madrid, Spain; [email protected] (S.G.-C.); [email protected] (Á.M.-R.); [email protected] (S.C.); [email protected] (A.C.-C.); [email protected] (L.M.F.); [email protected] (J.A.C.); Network Center for Biomedical Research on Obesity and Nutrition, Instituto de Salud Carlos III (CIBEROBN, ISCIII), E-28009 Madrid, Spain; IMDEA Food Institute, CEI UAM + CSIC, E-28049 Madrid, Spain 
 Department of Endocrinology, Hospital Infantil Universitario Niño Jesús, Instituto de Investigación La Princesa, E-28009 Madrid, Spain; [email protected] (S.G.-C.); [email protected] (Á.M.-R.); [email protected] (S.C.); [email protected] (A.C.-C.); [email protected] (L.M.F.); [email protected] (J.A.C.); Network Center for Biomedical Research on Obesity and Nutrition, Instituto de Salud Carlos III (CIBEROBN, ISCIII), E-28009 Madrid, Spain; Department of Pediatrics, Faculty of Medicine, Universidad Autónoma de Madrid, E-28029 Madrid, Spain; IMDEA Food Institute, CEI UAM + CSIC, E-28049 Madrid, Spain 
First page
1465
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
22279059
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2693933506
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.