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Abstract
The fall armyworm (Spodoptera frugiperda) is a highly polyphagous lepidopteran pest of relevant food and fiber staple crops. In the Americas, transgenic corn and cotton producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) have controlled and reduced the damage caused by S. frugiperda. However, cases of field-evolved S. frugiperda resistance to Bt corn producing the Cry1F insecticidal protein have been documented in North and South America. When characterized, field resistance to Cry1F is linked to insertions and mutations resulting in a modified or truncated ABC transporter subfamily C2 (SfABCC2) protein that serves as Cry1F receptor in susceptible S. frugiperda. In this work, we present detection of a large genomic deletion (~ 8 kb) affecting the SfABCC2 and an ABC transporter gene subfamily 3 –like gene (SfABCC3) as linked to resistance to Cry1F corn in a S. frugiperda strain from Florida (FL39). Monitoring for this genomic deletion using a discriminatory PCR reaction in field-collected S. frugiperda moths detected individuals carrying this allele in Florida, but not in surrounding states. This is the first report of a large genomic deletion being involved in resistance to a Bt insecticidal protein.
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Details
1 University of Tennessee, Department of Entomology and Plant Pathology, Knoxville, USA (GRID:grid.411461.7) (ISNI:0000 0001 2315 1184); University of Tennessee, Genome Science and Technology Graduate Program, Knoxville, USA (GRID:grid.411461.7) (ISNI:0000 0001 2315 1184)
2 University of Tennessee, Department of Entomology and Plant Pathology, Knoxville, USA (GRID:grid.411461.7) (ISNI:0000 0001 2315 1184)
3 Louisiana State University Agricultural Center, Department of Entomology, Baton Rouge, USA (GRID:grid.250060.1) (ISNI:0000 0000 9070 1054)
4 USDA-ARS Center for Medical, Agricultural and Veterinary Entomology, Insect Behavior and Biocontrol Research Unit, Gainesville, USA (GRID:grid.414781.f) (ISNI:0000 0000 9292 4307)
5 University of Georgia, Department of Entomology, Griffin, USA (GRID:grid.213876.9) (ISNI:0000 0004 1936 738X)
6 USDA-ARS Crop Genetics and Breeding Research Unit, Tifton, USA (GRID:grid.508985.9)
7 Clemson University, Pee Dee Research and Education Center, Plant and Environmental Sciences Department, Florence, USA (GRID:grid.26090.3d) (ISNI:0000 0001 0665 0280)