Abstract

The female reproductive tract (female-RT) must decipher the repertoire of molecular cues received from the male during copulation in order to activate and coordinate tract functionality necessary for high fertility. In Drosophila, this modulation is partially driven by spermathecal secretory cells (SSC). The SSC are a layer of cuboidal secretory glandular cells surrounding the spermatheca capsule where sperm is stored. It is unclear, however, how the SSC regulate the system’s activity. Here we show that mating activates the secretory machinery of the SSC. The SSC release a heterogeneous population of extracellular vesicles (EVs) which is involved in initiating and managing the increase in egg-laying, and possibly sperm storage. Moreover, sperm and male accessory gland proteins are essential for such mating-mediated SSC activity. Thus, mating regulates secretory/endocytic pathways required for trafficking of vesicles to SSC-female-RT target sites, which modulate and coordinate reproductive tract activity to achieve high fertility.

Visualisation of spermathecal secretory cell (SSC) secretion in Drosophila reveals that the SSC produces extracellular vesicles to modulate and coordinate reproductive tract activity.

Details

Title
Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila
Author
Sanchez-Lopez, Javier Arturo 1   VIAFID ORCID Logo  ; Twena, Shai 1 ; Apel, Ido 1 ; Kornhaeuser, Shani Chen 1   VIAFID ORCID Logo  ; Chasnitsky, Michael 2 ; Miklosi, Andras G. 3 ; Vega-Dominguez, Perla J. 3 ; Shephard, Alex 4 ; Hefetz, Amir 5 ; Heifetz, Yael 1   VIAFID ORCID Logo 

 The Hebrew University of Jerusalem, Department of Entomology, Rehovot, Israel (GRID:grid.9619.7) (ISNI:0000 0004 1937 0538) 
 The Hebrew University of Jerusalem, Institute of Biochemistry, Food Science and Nutrition, Rehovot, Israel (GRID:grid.9619.7) (ISNI:0000 0004 1937 0538) 
 ONI (Oxford Nanoimaging), Oxford, UK (GRID:grid.512103.4) 
 NanoView Biosciences, Malvern Hills Science Park, Malvern, UK (GRID:grid.512103.4) 
 DataGraph, Holon, Israel (GRID:grid.512103.4) 
Publication year
2022
Publication date
2022
Publisher
Nature Publishing Group
e-ISSN
23993642
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2701622241
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.