Abstract

Background

Browning of white adipose tissue (WAT) is a particularly appealing target for therapeutics in the treatment of obesity and related metabolic diseases. Although small extracellular vesicles (sEVs) released from adipose tissue (sEVs-AT) have emerged as novel player that regulate systemic metabolism by connecting different organs, the role of specific contents in sEVs-AT played in WAT browning has not been clarified.

Results

We revealed Nucleophosmin3 (NPM3), which was mainly transferred by sEVs derived from brown adipose tissue (sEVs-BAT), was served as a batokine that could induce WAT browning by regulating the stability of PRDM16 mRNA. sEVs-BAT enhanced the expressions of browning related genes in 3T3-L1 preadipocytes and WAT while knocking down of NPM3 in BAT impaired sEVs-BAT mediated WAT browning and weight loss in obesity.

Conclusion

These data provided new insight into the role of NPM3 in regulating the browning of WAT. Our study indicated that a supplement of sEVs-BAT might represent a promising therapeutic strategy to promote thermogenesis and energy expenditure in the future.

Details

Title
Nucleophosmin3 Carried by Small Extracellular Vesicles Contribute to White Adipose Tissue Browning
Author
Zhang, Yan; Yu, Mei  VIAFID ORCID Logo  ; Jia Dong; Wu, Yue; Tian, Weidong
Publication year
2022
Publication date
Jan 25, 2022
Publisher
Research Square
Source type
Working Paper
Language of publication
English
DOI
https://doi.org/10.21203/rs.3.rs-1245416/v1
ProQuest document ID
2705457734
Copyright
© 2022. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.