Full text

Turn on search term navigation

© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Neonates have a limited adaptive response of plasma cells, germinal center (GC) B cells, and T follicular helper cells (TFH). As neonatal vaccination can be an important tool for AIDS prevention, these limitations need to be overcome. Chimeric DNA vaccine encoding p55Gag HIV-1 protein conjugated with lysosomal-associated membrane protein 1 (LAMP-1) has been described as immunogenic in the neonate period. Herein, we investigated the immunologic mechanisms involved in neonatal immunization with a LAMP-1/p55Gag (LAMP/Gag) DNA vaccine in a C57BL/6 mouse background. Neonatal LAMP/Gag vaccination induced strong Gag-specific T-cell response until adulthood and elevated levels of anti-Gag IgG antibodies. We also demonstrated for the first time that the immunogenicity of the neonatal period with LAMP/Gag is due to the induction of high-affinity anti-p24 IgG antibodies and long-term plasma cells. Together with that, there is the generation of early TFH cells and the formation of GC sites with the upregulation of activation-induced cytidine deaminase (AID) enzyme mRNA and protein expression in draining lymph nodes after neonatal LAMP/Gag vaccination. These findings underscore that the LAMP-1 strategy in the chimeric vaccine could be useful to enhance antibody production even in the face of neonatal immaturity, and they contribute to the development of new vaccine approaches for other emerging pathogens at an early stage of life.

Details

Title
LAMP-1 Chimeric to HIV-1 p55Gag in the Immunization of Neonate Mice Induces an Early Germinal Center Formation and AID Expression
Author
Franciane Mouradian Emidio Teixeira 1   VIAFID ORCID Logo  ; Luana de Mendonça Oliveira 1 ; Pietrobon, Anna Julia 1 ; Érika Machado de Salles 2 ; Maria Regina D’Império Lima 2 ; Isabelle Freire Tabosa Viana 3   VIAFID ORCID Logo  ; Roberto Dias Lins 3   VIAFID ORCID Logo  ; Rigato, Paula Ordonhez 4   VIAFID ORCID Logo  ; Ernesto Torres de Azevedo Marques 5   VIAFID ORCID Logo  ; Alberto José da Silva Duarte 6 ; Maria Notomi Sato 1   VIAFID ORCID Logo 

 Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Tropical Medicine Institute of São Paulo, University of São Paulo Medical School, São Paulo 05403000, Brazil; [email protected] (F.M.E.T.); [email protected] (L.d.M.O.); [email protected] (A.J.P.); [email protected] (A.J.d.S.D.); Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508000, Brazil; [email protected] (É.M.d.S.); [email protected] (M.R.D.L.) 
 Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508000, Brazil; [email protected] (É.M.d.S.); [email protected] (M.R.D.L.) 
 Department of Virology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife 50740465, Brazil; [email protected] (I.F.T.V.); [email protected] (R.D.L.); [email protected] (E.T.d.A.M.) 
 Laboratory of Immunobiology and Biomarkers, Center of Immunology, Institute Adolfo Lutz, São Paulo 01246000, Brazil; [email protected] 
 Department of Virology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife 50740465, Brazil; [email protected] (I.F.T.V.); [email protected] (R.D.L.); [email protected] (E.T.d.A.M.); Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA 15213, USA 
 Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Tropical Medicine Institute of São Paulo, University of São Paulo Medical School, São Paulo 05403000, Brazil; [email protected] (F.M.E.T.); [email protected] (L.d.M.O.); [email protected] (A.J.P.); [email protected] (A.J.d.S.D.) 
First page
1246
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
2076393X
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2706286951
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.