Abstract

Coronavirus disease 2019 (COVID-19) continues to significantly impact the global population, thus countermeasure platforms that enable rapid development of therapeutics against variants of SARS-CoV-2 are essential. We report use of a phage display human antibody library approach to rapidly identify neutralizing antibodies (nAbs) against SARS-CoV-2. We demonstrate the binding and neutralization capability of two nAbs, STI-2020 and STI-5041, against the SARS-CoV-2 WA-1 strain as well as the Alpha and Beta variants. STI-2020 and STI-5041 were protective when administered intravenously or intranasally in the golden (Syrian) hamster model of COVID-19 challenged with the WA-1 strain or Beta variant. The ability to administer nAbs intravenously and intranasally may have important therapeutic implications and Phase 1 healthy subjects clinical trials are ongoing.

Details

Title
Unbiased approach to identify and assess efficacy of human SARS-CoV-2 neutralizing antibodies
Author
Cao, Xia 1 ; Maruyama, Junki 2 ; Zhou, Heyue 1 ; Fu, Yanwen 1 ; Kerwin, Lisa 1 ; Powers, Colin 1 ; Sattler, Rachel A. 2 ; Manning, John T. 2 ; Singh, Alok 1 ; Lim, Reyna 1 ; Healy, Laura D. 1 ; Johnson, Sachi 1 ; Paz Cabral, Elizabeth 1 ; Li, Donghui 1 ; Lu, Lucy 1 ; Ledesma, Arthur 1 ; Lee, Daniel 1 ; Richards, Susan 1 ; Rivero-Nava, Laura 1 ; Li, Yan 1 ; Shen, Weiqun 1 ; Stegman, Karen 1 ; Blair, Benjamin 1 ; Urata, Shinji 2 ; Kishimoto-Urata, Magumi 2 ; Ko, Jamie 1 ; Du, Na 1 ; Morais, Kyndal 1 ; Lawrence, Kate 1 ; Rivera, Ianne 1 ; Pai, Chin-I. 1 ; Bresson, Damien 1 ; Brunswick, Mark 1 ; Zhang, Yanliang 1 ; Ji, Henry 1 ; Paessler, Slobodan 2 ; Allen, Robert D. 1 

1  Sorrento Therapeutics, Inc., San Diego, USA (GRID:grid.430349.9) (ISNI:0000 0004 5998 8172) 
2  University of Texas Medical Branch, Galveston National Laboratory, Department of Pathology, Galveston, USA (GRID:grid.176731.5) (ISNI:0000 0001 1547 9964) 
Publication year
2022
Publication date
2022
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-022-19780-7
ProQuest document ID
2714546404
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.