Development and radiolabeling of lipid nanoparticles with [99mTc]Tc-HMPAO: Characterization, stability, cytotoxicity and cell binding studies

Abstract

In this study, we aimed to develop new lipid based nanoparticles (LPNs) and radiolabeled LPNs with [^99mTc]Tc-HMPAO to investigate its cell binding capacity comparatively with [sup>99mTc]Tc-HMPAO on different cancer cells. According to obtained results, LPNs with zeta potential of -27.4 ± 0.95 mV, particle size of 93.5 ± 1.17 nm, and polydispersity index of 0.35 ± 0.04 were successfully developed. The optimum radiolabeling efficiency was found to be above 90% at 15-min of incubation time. The cell binding capacity of [^99mTc]Tc-HMPAO-LPNs was found to be higher than [^99mTc]Tc-HMPAO in cancer cell lines. The results demonstrated that [^99mTc]Tc-HMPAO-LPNs may be a promising agent for cancer diagnosis alternatively to [^99mTc]Tc-HMPAO.