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Abstract
Cephalopods have been considered enigmatic animals that have attracted the attention of scientists from different areas of expertise. However, there are still many questions to elucidate the way of life of these invertebrates. The aim of this study is to construct a reference transcriptome in Octopus vulgaris early life stages to enrich existing databases and provide a new dataset that can be reused by other researchers in the field. For that, samples from different developmental stages were combined including embryos, newly-hatched paralarvae, and paralarvae of 10, 20 and 40 days post-hatching. Additionally, different dietary and rearing conditions and pathogenic infections were tested. At least three biological replicates were analysed per condition and submitted to RNA-seq analysis. All sequencing reads from experimental conditions were combined in a single dataset to generate a reference transcriptome assembly that was functionally annotated. The number of reads aligned to this reference was counted to estimate the transcript abundance in each sample. This dataset compiled a complete reference for future transcriptomic studies in O. vulgaris.
Measurement(s) | Transcriptome sequencing assay |
Technology Type(s) | RNA-seq assay (Illumina) |
Sample Characteristic - Organism | Octopus vulgaris |
Sample Characteristic - Environment | Ocean |
Sample Characteristic - Location | NW Spain (Ría de Vigo, Galicia) |
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1 Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain (GRID:grid.419099.c) (ISNI:0000 0001 1945 7711)
2 Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain (GRID:grid.419099.c) (ISNI:0000 0001 1945 7711); Pescanova Biomarine Center, O Grove, Spain (GRID:grid.419099.c)
3 Centro Oceanográfico de Vigo (COV-IEO), CSIC, Vigo, Spain (GRID:grid.4711.3) (ISNI:0000 0001 2183 4846); Pescanova Biomarine Center, O Grove, Spain (GRID:grid.4711.3)
4 Centro Oceanográfico de Vigo (COV-IEO), CSIC, Vigo, Spain (GRID:grid.4711.3) (ISNI:0000 0001 2183 4846)
5 Centro Oceanográfico de Canarias (COC-IEO), CSIC. Calle La Farola del Mar n° 22, Dársena Pesquera, Santa Cruz de Tenerife, Spain (GRID:grid.4711.3)
6 Instituto de Acuicultura de Torre de la Sal (IATS), CSIC. Torre de la Sal s/n, Ribera de Cabanes, Spain (GRID:grid.452499.7) (ISNI:0000 0004 1800 9433)