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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Semiconducting polymer dots (Pdots) are rapidly becoming one of the most studied nanoparticles in fluorescence bioimaging and sensing. Their small size, high brightness, and resistance to photobleaching make them one of the most attractive fluorophores for fluorescence imaging and sensing applications. This paper highlights our recent advances in fluorescence bioimaging and sensing with nanoscale luminescent Pdots, specifically the use of organic dyes as dopant molecules to modify the optical properties of Pdots to enable deep red and near infrared fluorescence bioimaging applications and to impart sensitivity of dye doped Pdots towards selected analytes. Building on our earlier work, we report the formation of secondary antibody-conjugated Pdots and provide Cryo-TEM evidence for their formation. We demonstrate the selective targeting of the antibody-conjugated Pdots to FLAG-tagged FLS2 membrane receptors in genetically engineered plant leaf cells. We also report the formation of a new class of luminescent Pdots with emission wavelengths of around 1000 nm. Finally, we demonstrate the formation and utility of oxygen sensing Pdots in aqueous media.

Details

Title
Near Infrared Emitting Semiconductor Polymer Dots for Bioimaging and Sensing
Author
Connor Riahin 1   VIAFID ORCID Logo  ; Mendis, Kushani 1 ; Busick, Brandon 1 ; Ptaszek, Marcin 1   VIAFID ORCID Logo  ; Yang, Mengran 2 ; Stacey, Gary 2 ; Parvate, Amar 3   VIAFID ORCID Logo  ; Evans, James E 4   VIAFID ORCID Logo  ; Traeger, Jeremiah 3 ; Hu, Dehong 3   VIAFID ORCID Logo  ; Orr, Galya 3 ; Rosenzweig, Zeev 1   VIAFID ORCID Logo 

 Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, MD 21250, USA 
 Divisions of Plant Sciences and Biochemistry, University of Missouri, Columbia, MO 65211, USA 
 Environmental Molecular Sciences Laboratory (EMSL), Pacific Northwest National Laboratory, Richland, WA 99354, USA 
 Environmental Molecular Sciences Laboratory (EMSL), Pacific Northwest National Laboratory, Richland, WA 99354, USA; School of Biological Sciences, Washington State University, Pullman, WA 99164, USA 
First page
7218
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
14248220
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2724306229
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.