Abstract

Exon junction complexes (EJCs) mark untranslated spliced mRNAs and are crucial for the mRNA lifecycle. An imbalance in EJC dosage alters mouse neural stem cell (mNSC) division and is linked to human neurodevelopmental disorders. In quiescent mNSC and immortalized human retinal pigment epithelial (RPE1) cells, centrioles form a basal body for ciliogenesis. Here, we report that EJCs accumulate at basal bodies of mNSC or RPE1 cells and decline when these cells differentiate or resume growth. A high-throughput smFISH screen identifies two transcripts accumulating at centrosomes in quiescent cells, NIN and BICD2. In contrast to BICD2, the localization of NIN transcripts is EJC-dependent. NIN mRNA encodes a core component of centrosomes required for microtubule nucleation and anchoring. We find that EJC down-regulation impairs both pericentriolar material organization and ciliogenesis. An EJC-dependent mRNA trafficking towards centrosome and basal bodies might contribute to proper mNSC division and brain development.

Exon junction complexes (EJCs) that mark untranslated mRNA are involved in transport, translation and nonsense-mediated mRNA decay. Here the authors show centrosomal localization of EJCs which appears to be required for both the localization of NIN mRNA around centrosomes and ciliogenesis.

Details

Title
Exon junction complex dependent mRNA localization is linked to centrosome organization during ciliogenesis
Author
Kwon, Oh Sung 1   VIAFID ORCID Logo  ; Mishra, Rahul 2   VIAFID ORCID Logo  ; Safieddine, Adham 3   VIAFID ORCID Logo  ; Coleno, Emeline 3 ; Alasseur, Quentin 1 ; Faucourt, Marion 1 ; Barbosa, Isabelle 1 ; Bertrand, Edouard 3   VIAFID ORCID Logo  ; Spassky, Nathalie 1 ; Le Hir, Hervé 1 

1  PSL Research University, Institut de Biologie de l’Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, Paris, France (GRID:grid.440907.e) (ISNI:0000 0004 1784 3645) 
2  PSL Research University, Institut de Biologie de l’Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, Paris, France (GRID:grid.440907.e) (ISNI:0000 0004 1784 3645); Czech Academy of Sciences, Institute of Parasitology, Biology Centre, Ceske Budejovice, Czech Republic (GRID:grid.418095.1) (ISNI:0000 0001 1015 3316) 
3  CNRS, Institut de Génétique Moléculaire de Montpellier, University of Montpellier, Montpellier, France (GRID:grid.4444.0) (ISNI:0000 0001 2112 9282); CNRS, Equipe labélisée Ligue Nationale Contre le Cancer, University of Montpellier, Montpellier, France (GRID:grid.4444.0) (ISNI:0000 0001 2112 9282) 
Publication year
2021
Publication date
2021
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-021-21590-w
ProQuest document ID
2727100193
Copyright
© The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.