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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

In this paper, a Second-Harmonic-Generation (SHG) microscope was used to study the relationship between collagen structural arrangement, image quality and polarization sensitivity in human corneas with different organizations. The degree of order (or alternatively, the Structural Dispersion, SD) was quantified using the structure tensor method. SHG image quality was evaluated with different objective metrics. Dependence with polarization was quantified by means of a parameter defined as polarimetric modulation, which employs polarimetric SHG images acquired with four independent polarization states. There is a significant exponential relationship between the quality of the SHG images and the SD of the samples. Moreover, polarization sensitivity strongly depends on collagen arrangement. For quasi- or partially organized specimens, there is a polarization state that noticeably improves the image quality, providing additional information often not seen in other SHG images. This does not occur in non-organized samples. This fact is closely related to polarimetric modulation, which linearly decreases with the SD. Understanding in more detail the relationships that take place between collagen distribution, image quality and polarization sensitivity brings the potential to enable the development of optimized SHG image acquisition protocols and novel objective strategies for the analysis and detection of pathologies related to corneal collagen disorders, as well as surgery follow-ups.

Details

Title
Collagen Organization, Polarization Sensitivity and Image Quality in Human Corneas using Second Harmonic Generation Microscopy
Author
Bueno, Juan M 1   VIAFID ORCID Logo  ; Martínez-Ojeda, Rosa M 1   VIAFID ORCID Logo  ; Yago, Inés 2 ; Ávila, Francisco J 3   VIAFID ORCID Logo 

 Laboratorio de Óptica, Instituto Universitario de Investigación en Óptica y Nanofísica, Universidad de Murcia, Campus de Espinardo (Ed. 34), 30100 Murcia, Spain 
 Servicio de Oftalmología, Hospital Virgen de la Arrixaca, 30120 Murcia, Spain 
 Department Física Aplicada, Universidad de Zaragoza, 50009 Zaragoza, Spain 
First page
672
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
23046732
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2728521318
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.