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Copyright © 2022 Shin-Kyu Lee et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0/

Abstract

Objective. Tooth extraction causes a wound with hard and soft tissue defects in the alveolar ridge. Few studies have reported the function of microRNAs (miRNAs) in the healing of extraction sockets. This study used bioinformatics analysis to reveal the possible relevance and role of miRNAs during the early stages following tooth extraction. Materials and Methods. Socket tissues from beagle dogs (Canis familiaris; two males and two females) were collected 1 and 12 hours after extraction of premolars on both sides of the mandible. miRNA expression was profiled through miRNA sequencing, and hub miRNAs showing characteristic expression patterns were selected and subjected to target enrichment analysis. Alkaline phosphatase (ALP) activity analysis and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were performed to verify the effect of hub miRNA on osteoblast differentiation and bone regeneration in vivo. Results. Five miRNAs were identified to have consistently high expression levels, with cfa-miR-451 showing the highest expression. Additionally, 20 hub miRNAs were selected as candidates expected to play an important role in the healing process. Pathways, such as the MAPK, axon guidance, TGF-β, and Wnt signaling, were significantly enriched. Among hub miRNAs, miR-190a-5p increased ALP activity and mRNA expression of osteogenic markers and increased new bone formation in vivo. Conclusions. Our findings suggest that miRNAs may be involved in the earliest stages of socket healing after tooth extraction and can play an important role in moderating the entire socket healing mechanism in the extraction socket.

Details

Title
miRNA-Based Early Healing Mechanism of Extraction Sockets: miR-190a-5p, a Potential Enhancer of Bone Healing
Author
Shin-Kyu, Lee 1   VIAFID ORCID Logo  ; Su-Hyeon, Jung 1   VIAFID ORCID Logo  ; Sang-Jin, Song 2   VIAFID ORCID Logo  ; Lee, In-Gyu 2   VIAFID ORCID Logo  ; Jae-Yoon, Choi 1   VIAFID ORCID Logo  ; Zadeh, Homayoun 3   VIAFID ORCID Logo  ; Dong-Woon, Lee 2   VIAFID ORCID Logo  ; Sung-Hee Pi 2   VIAFID ORCID Logo  ; Hyung-Keun You 1   VIAFID ORCID Logo 

 Department of Periodontology, School of Dentistry, Wonkwang University, Iksan, Jeonbuk, Republic of Korea; Clinical Lab for Innovative Periodontology, Department of Periodontology, School of Dentistry, Wonkwang University, Iksan, Jeonbuk, Republic of Korea 
 Department of Periodontology, School of Dentistry, Wonkwang University, Iksan, Jeonbuk, Republic of Korea 
 VISTA Institute for Therapeutic Innovations, Woodland Hills, CA, USA 
Editor
Saber Khazaei
Publication year
2022
Publication date
2022
Publisher
John Wiley & Sons, Inc.
ISSN
23146133
e-ISSN
23146141
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2730156933
Copyright
Copyright © 2022 Shin-Kyu Lee et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0/