Abstract

The endophytic fungus Paecilomyces sp. (AUMC 15510) was isolated from healthy stem samples of the Egyptian medicinal plant Cornulaca monacantha. We used GC–MS and HPLC analysis to identify the bioactive constituents of ethyl acetate crude extract of Paecilomyces sp. (PsEAE). Six human microbial pathogens have been selected to evaluate the antimicrobial activity of PsEAE. Our data showed that the extract has significant antimicrobial activity against all tested pathogens. However, the best inhibitory effect was observed against Bacillus subtilis ATCC 6633 and Pseudomonas aeruginosa ATCC 90274 with a minimum inhibitory concentration (MIC) of 3.9 μg/ml and minimum bactericidal concentration (MBC) of 15.6 μg/ml, for both pathogens. Also, PsEAE exerts a significant inhibition on the biofilm formation of the previously mentioned pathogenic strains. In addition, we evaluated the wound healing efficiency of PsEAE on earthworms (Lumbricus castaneus) as a feasible and plausible model that mimics human skin. Interestingly, PsEAE exhibited a promising wound healing activity and enhanced wound closure. In conclusion, Paecilomyces sp. (AUMC 15510) could be a sustainable source of antimicrobial agents and a potential therapeutic target for wound management.

Details

Title
The antimicrobial, antibiofilm, and wound healing properties of ethyl acetate crude extract of an endophytic fungus Paecilomyces sp. (AUMC 15510) in earthworm model
Author
Salem, Shimaa H. 1 ; El-Maraghy, Saad S. 1 ; Abdel-Mallek, Ahmed Y. 2 ; Abdel-Rahman, Mohamed A. A. 3 ; Hassanein, Emad H. M. 4 ; Al-Bedak, Osama A. 5 ; El-Aziz, Fatma El-Zahraa A. Abd 6   VIAFID ORCID Logo 

1  Assiut University, Fungal Physiology Laboratory, Botany and Microbiology Department, Faculty of Science, Assiut, Egypt (GRID:grid.252487.e) (ISNI:0000 0000 8632 679X) 
2  Assiut University, Mycology Laboratory, Botany and Microbiology Department, Faculty of Science, Assiut, Egypt (GRID:grid.252487.e) (ISNI:0000 0000 8632 679X) 
3  Agricultural Research Center, Plant Protection Research Institute, Dokki, Egypt (GRID:grid.418376.f) (ISNI:0000 0004 1800 7673) 
4  Al-Azhar University-Assiut Branch, Department of Pharmacology and Toxicology, Faculty of Pharmacy, Assiut, Egypt (GRID:grid.411303.4) (ISNI:0000 0001 2155 6022) 
5  Assiut University, Assiut University Mycological Centre (AUMC), Assiut, Egypt (GRID:grid.252487.e) (ISNI:0000 0000 8632 679X) 
6  Assiut University, Department of Zoology, Faculty of Science, Assiut, Egypt (GRID:grid.252487.e) (ISNI:0000 0000 8632 679X) 
Publication year
2022
Publication date
2022
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-022-23831-4
ProQuest document ID
2734857385
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.