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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Aldosterone-producing adenomas (APAs) have different steroid profiles in serum, depending on the causative genetic mutation. Ion mobility is a separation technique for gas-phase ions based on their m/z values, shapes, and sizes. Human serum (100 µL) was purified by liquid–liquid extraction using tert-butyl methyl ether/ethyl acetate at 1/1 (v/v) and mixed with deuterium-labeled steroids as the internal standard. The separated supernatant was dried, re-dissolved in water containing 20% methanol, and injected into a liquid chromatography–ion mobility–mass spectrometer (LC/IM/MS). We established a highly sensitive assay system by separating 20 steroids based on their retention time, m/z value, and drift time. Twenty steroids were measured in the serum of patients with primary aldosteronism, essential hypertension, and healthy subjects and were clearly classified using principal component analysis. This method was also able to detect phosphatidylcholine and phosphatidylethanolamine, which were not targeted. LC/IM/MS has a high selectivity for known compounds and has the potential to provide information on unknown compounds. This analytical method has the potential to elucidate the pathogenesis of APA and identify unknown steroids that could serve as biomarkers for APA with different genetic mutations.

Details

Title
Comprehensive Steroid Assay with Non-Targeted Analysis Using Liquid Chromatography Ion Mobility Mass Spectrometry
Author
Yamakawa, Mai 1 ; Karashima, Shigehiro 2   VIAFID ORCID Logo  ; Takata, Riko 3 ; Haba, Taichi 4 ; Kuroiwa, Keigo 5 ; Touyama, Hideaki 6 ; Hashimoto, Atsushi 7 ; Konishi, Seigo 8 ; Aono, Daisuke 8 ; Kometani, Mitsuhiro 8   VIAFID ORCID Logo  ; Nambo, Hidetaka 9 ; Yoneda, Takashi 10   VIAFID ORCID Logo  ; Osaka, Issey 11 

 Department of Biotechnology and Pharmaceutical Engineering, Graduate School of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan 
 Institute of Liberal Arts and Science, Kanazawa University, Kanazawa 920-1192, Japan 
 Department of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan 
 Department of Electronics and Information Engineering, Graduate School of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan 
 Department of Electronics and Information Engineering, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan 
 Department of Electronics and Information Engineering, Graduate School of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan; Department of Information Systems Engineering, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan 
 Faculty of Transdisciplinary Sciences, Institute of Transdisciplinary Sciences, Kanazawa University, Kanazawa 920-1192, Japan 
 Department of Endocrinology and Metabolism, Graduate School of Medicine, Kanazawa University, Kanazawa 920-8641, Japan 
 School of Electrical Information Communication Engineering, College of Science and Engineering, Kanazawa University, Kanazawa 920-1192, Japan 
10  Faculty of Transdisciplinary Sciences, Institute of Transdisciplinary Sciences, Kanazawa University, Kanazawa 920-1192, Japan; Department of Endocrinology and Metabolism, Graduate School of Medicine, Kanazawa University, Kanazawa 920-8641, Japan; Department of Health Promotion and Medicine of the Future, Graduate School of Medicine, Kanazawa University, Kanazawa 920-8541, Japan 
11  Department of Biotechnology and Pharmaceutical Engineering, Graduate School of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan; Department of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan 
First page
13858
Publication year
2022
Publication date
2022
Publisher
MDPI AG
ISSN
16616596
e-ISSN
14220067
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2739445057
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.