Temporal landscape of mitochondrial proteostasis governed by the UPRmt

Abstract

Breakdown of mitochondrial proteostasis activates quality control pathways including the mitochondrial unfolded protein response (UPR^mt) and PINK1/Parkin mitophagy. However, beyond the upregulation of chaperones and proteases, we have a limited understanding of how the UPR^mt remodels and restores damaged mito-proteomes. Here, we have developed a functional proteomics framework, termed MitoPQ (Mitochondrial Proteostasis Quantification), to dissect the UPR^mts role in maintaining proteostasis during stress. We discover essential roles for the UPR^mt in both protecting and repairing proteostasis, with oxidative phosphorylation metabolism being a central target of the UPR^mt. Transcriptome analyses together with MitoPQ reveal that UPR^mt transcription factors drive independent signaling arms that act in concert to maintain proteostasis. Unidirectional interplay between the UPR^mt and PINK1/Parkin mitophagy was found to promote oxidative phosphorylation recovery when the UPR^mt failed. Collectively, this study defines the network of proteostasis mediated by the UPR^mt and highlights the value of functional proteomics in decoding stressed proteomes.

Competing Interest Statement

Michael Lazarou is a member of the scientific advisory board of Automera.