Molecular genetic differentiation of native populations of Mediterranean blue mussels, Mytilus galloprovincialis Lamarck, 1819, and the relationship with environmental variables

Abstract

Blue mussels of the genus Mytilus are important ecosystem engineers in intertidal and subtidal communities. The distribution of Mytilus mussels is influenced by a number of benthic and pelagic environmental variables (e.g., substratum type and availability, water movement, phytoplankton production, physical disturbance) as well as interactions between these variables. Because of its broad tolerance of environmental variation the Mediterranean species, Mytilus galloprovincialis, has the greatest ability of all blue mussels to colonise new geographic regions. Understanding how population genetic variation is related to, or caused by, environmental variation is important but has long been a challenge. The present study examined the genetic differentiation of native populations of M. galloprovincialis throughout its entire geographic range in the Mediterranean Sea, the Black Sea and the Sea of Azov using 53 single nucleotide polymorphisms (SNP loci). Mussels, in total 1004 individuals collected from 36 locations, were genotyped and combined with existing SNP data for mussels from 11 reference sites. Pairwise comparisons of F_ST values, correspondence analysis (CA) and STRUCTURE analysis all revealed four groups of populations: the Atlantic Ocean; the western Mediterranean; the Aegean Sea; and the Azov, Black and Marmara Seas. One population – from Algeria (Oran West) – was intermediate between the two main groups of the Mediterranean Sea and Atlantic Ocean. Seascape genetic analyses using GLM and DistLM analyses were employed to test site-specific genetic variation as a function of 13 environmental variables. The GLM identified five environmental variables that explained variation in site-specific F_ST values, whilst in the DistLM best-fit model only four were significant. These analyses suggest that a complex mix of environmental variables contribute to explaining genetic variation of M. galloprovincialis populations within the Mediterranean Sea, which most likely reflects the complex geological history of formation, isolation and reconnection among the regional sub-basins of the Sea.