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Abstract
Aspergillus terreus is well-known for lovastatin and itaconic acid production with biomedical and commercial importance. The mechanisms of metabolite formation have been extensively studied to improve their yield through genetic engineering. However, the combined repertoire of carbohydrate-active enzymes (CAZymes), cytochrome P450s (CYP) enzymes, and secondary metabolites (SMs) in the different A. terreus strains has not been well studied yet, especially with respect to the presence of biosynthetic gene clusters (BGCs). Here we present a 30 Mb whole genome sequence of A. terreus ATCC 20541 in which we predicted 10,410 protein-coding genes. We compared the CAZymes, CYPs enzyme, and SMs across eleven A. terreus strains, and the results indicate that all strains have rich pectin degradation enzyme and CYP52 families. The lovastatin BGC of lovI was linked with lovF in A. terreus ATCC 20541, and the phenomenon was not found in the other strains. A. terreus ATCC 20541 lacked a non-ribosomal peptide synthetase (AnaPS) participating in acetylaszonalenin production, which was a conserved protein in the ten other strains. Our results present a comprehensive analysis of CAZymes, CYPs enzyme, and SM diversities in A. terreus strains and will facilitate further research in the function of BGCs associated with valuable SMs.
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1 National Sun Yat-Sen University, Department of Marine Biotechnology and Resources, Kaohsiung City, Taiwan (GRID:grid.412036.2) (ISNI:0000 0004 0531 9758)
2 Technical University of Denmark, DTU Bioengineering, Kgs. Lyngby, Denmark (GRID:grid.5170.3) (ISNI:0000 0001 2181 8870)
3 National Sun Yat-Sen University, Department of Marine Biotechnology and Resources, Kaohsiung City, Taiwan (GRID:grid.412036.2) (ISNI:0000 0004 0531 9758); Kaohsiung Medical University, Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung City, Taiwan (GRID:grid.412019.f) (ISNI:0000 0000 9476 5696)
4 National Sun Yat-Sen University, Department of Marine Biotechnology and Resources, Kaohsiung City, Taiwan (GRID:grid.412036.2) (ISNI:0000 0004 0531 9758); National Sun Yat-Sen University, Doctoral Degree Program in Marine Biotechnology, Kaohsiung City, Taiwan (GRID:grid.412036.2) (ISNI:0000 0004 0531 9758)