Full text

Turn on search term navigation

© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Most gluten analysis methods have been developed to detect intact gluten, but they have shown limitations in certain foods and beverages in which gluten proteins are hydrolyzed. Methods based on G12/A1 moAbs detect the sequences of gluten immunogenic peptides (GIP), which are the main contributors to the immune response of celiac disease (CD). Immunogenic sequences with tandem epitopes for G12/A1 have been found in beers with <20 mg/kg gluten, which could be consumed by CD patients according to the Codex Alimentarius. Therefore, an accurate method for the estimation of the immunogenicity of a beer is to use two moAbs that can recognize celiac T cell epitopes comprising most of the immunogenic response. Here, a specific and sensitive method based on G12/A1 LFIA was developed to detect GIP in beers labeled gluten-free or with low gluten content, with an LOD of 0.5 mg/kg. A total of 107 beers were analyzed, of those 6.5% showed levels higher than 20 mg/kg gluten and 29% showed levels above the LOD. In addition, G12/A1 LFIA detected gluten in 15 more beer samples than competitive ELISA with another antibody. Despite their labeling, these beers contained GIP which may cause symptoms and/or intestinal damage in CD patients.

Details

Title
A Highly Sensitive Method for the Detection of Hydrolyzed Gluten in Beer Samples Using LFIA
Author
Segura, Verónica 1   VIAFID ORCID Logo  ; Siglez, Miguel Ángel 2 ; Ruiz-Carnicer, Ángela 1   VIAFID ORCID Logo  ; Martín-Cabrejas, Izaskun 3 ; van der Hofstadt, María 4 ; Mellado, Encarnación 1 ; Comino, Isabel 1   VIAFID ORCID Logo  ; Sousa, Carolina 1   VIAFID ORCID Logo 

 Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain 
 Biomedal S.L., 41900 Camas, Spain 
 Food Technology Department, Veterinary Faculty, Complutense University of Madrid, 28040 Madrid, Spain 
 ALINUA, Food and Nutrition Cabinet Health Science Faculty, University of Alicante, UA, 03690 Alicante, Spain 
First page
160
Publication year
2023
Publication date
2023
Publisher
MDPI AG
e-ISSN
23048158
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2761175141
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.