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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Simple Summary

The Diamondback moth (DBM, Plutella xylostella) is a worldwide destructive pest with a typical phototaxis, mainly damaging cruciferous vegetables. Our previous study revealed that an LW-opsin mutation causes a defective phototaxis in P. xylostella, but the mechanism behind this phenomenon remains unknown. In this study, the head transcriptomes of the male Geneva 88 strain of P. xylostella (G88) and an LW-opsin mutant were compared to reveal the expression changes of other genes in the phototransduction pathway caused by the mutation of LW-opsin. The results showed that the LW-opsin mutation caused expression changes of the genes in the phototransduction pathway, such as neither inactivation nor afterpotential C (ninaC), retinal degeneration C (rdgC), arrestin1 (arr1), cryptochrome1 (cry1), transient receptor potential (trp), transient receptor potential like (trpl) and inactivation nor afterpotential D (inaD). Considering CRY1 acts as a UV/blue-light photoreceptor, the influence of a cry1 mutation on the phototaxis of P. xylostella was examined, and the results showed that the male cry1 mutant possessed higher phototactic rates to UV and blue lights than the male G88. Our results provide a foundation for further exploration of the phototransduction pathway of P. xylostella.

Abstract

Plutella xylostella is a typical phototactic pest. LW-opsin contributes to the phototaxis of P. xylostella, but the expression changes of other genes in the phototransduction pathway caused by the mutation of LW-opsin remain unknown. In the study, the head transcriptomes of male G88 and LW-opsin mutants were compared. A GO-function annotation showed that DEGs mainly belonged to the categories of molecular functions, biological processes, and cell composition. Additionally, a KEGG-pathway analysis suggested that DEGs were significantly enriched in some classical pathways, such as the phototransduction-fly and vitamin digestion and absorption pathways. The mRNA expressions of genes in the phototransduction-fly pathway, such as Gq, ninaC, and rdgC were significantly up-regulated, and trp, trpl, inaD, cry1, ninaA and arr1 were significantly down-regulated. The expression trends of nine DEGs in the phototransduction pathway confirmed by a RT-qPCR were consistent with transcriptomic data. In addition, the influence of a cry1 mutation on the phototaxis of P. xylostella was examined, and the results showed that the male cry1 mutant exhibited higher phototactic rates to UV and blue lights than the male G88. Our results indicated that the LW-opsin mutation changed the expression of genes in the phototransduction pathway, and the mutation of cry1 enhanced the phototaxis of a P. xylostella male, providing a basis for further investigation on the phototransduction pathway in P. xylostella.

Details

Title
An LW-Opsin Mutation Changes the Gene Expression of the Phototransduction Pathway: A Cryptochrome1 Mutation Enhances the Phototaxis of Male Plutella xylostella (Lepidoptera: Plutellidae)
Author
Shao-Ping, Chen 1 ; Xiao-Lu, Lin 2 ; Rong-Zhou, Qiu 3 ; Mei-Xiang, Chi 3 ; Yang, Guang 2   VIAFID ORCID Logo 

 State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou 350002, China; Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China; Key Laboratory of Green Pest Control, Fujian Province University, Fuzhou 350002, China; Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China 
 State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou 350002, China; Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China; Key Laboratory of Green Pest Control, Fujian Province University, Fuzhou 350002, China 
 Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China 
First page
72
Publication year
2023
Publication date
2023
Publisher
MDPI AG
e-ISSN
20754450
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2767220409
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.