Identification of genes associated with abiotic stress tolerance in sweetpotato using weighted gene co-expression network analysis

Abstract

Sweetpotato, Ipomoea batatas (L.), a key food security crop, is negatively impacted by heat, drought, and salinity stress. We exposed the orange-fleshed cultivar, Beauregard, to 24 and 48 hours of heat and salt stresses to identify differentially expressed genes (DEGs) in leaves. Analysis revealed both shared and unique sets of up-regulated (650 for heat; 287 for salt) and down-regulated (1,249 for heat; 793 for salt) DEGs suggesting common, yet stress-specific transcriptional responses to these two abiotic stressors. Gene Ontology analysis of downregulated DEGs common to both heat and salt stress revealed enrichment of terms associated with cell population proliferation suggestive of an impact on the cell cycle by the heat stress. To identify shared and unique gene coexpression networks under multiple abiotic stress conditions, weighted gene co-expression network analysis was performed using gene expression profiles from heat, salt, and drought stress treated Beauregard leaves yielding 18 coexpression modules. One module was enriched for response to water deprivation, response to abscisic acid, and nitrate transport indicating synergetic crosstalk between nitrogen, water and phytohormones with genes encoding osmotin, cell expansion, and cell wall modification proteins present as key hub genes in this drought-associated module. This research lays the background for future research in mediating abiotic stress tolerance in sweetpotato.

Competing Interest Statement

The authors have declared no competing interest.