Abstract

As a highly conserved and ubiquitously expressed serine/threonine kinase, p21-activated kinase 2 (PAK2) participates in diverse biologic events. However, its roles in mouse oocyte meiotic maturation remain unclear. The present study revealed that mouse oocytes depleted of Pak2 were unable to completely progress through meiosis and that a majority were arrested at metaphase I. Pak2 depletion thus prompted MI arrest and induced meiotic chromosome alignment defects in mouse oocytes, in part due to a reduction in polo-like kinase (PLK1). We demonstrated that PAK2’s interaction with PLK1 protected it from degradation by APC/CCdh1, and that it promoted meiotic progression and bipolar spindle formation. Our data collectively display critical functions for PAK2 in meiotic progression and chromosome alignment in mouse oocytes.

Details

Title
PAK2 is essential for chromosome alignment in metaphase I oocytes
Author
Zeng, Juan 1   VIAFID ORCID Logo  ; Wang, Shiwei 2   VIAFID ORCID Logo  ; Gao, Min 3 ; Lu, Dian 4 ; Song, Shuang 2 ; Chen, Diyu 4 ; Fan, Weimin 4 ; Xu, Zhiliang 4 ; Zhang, Zhiguo 5   VIAFID ORCID Logo  ; Sun, Xiaofang 4   VIAFID ORCID Logo 

1  The Third Affiliated Hospital of Guangzhou Medical University, Department of Obstetrics and Gynecology, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou, China (GRID:grid.417009.b) (ISNI:0000 0004 1758 4591); Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China (GRID:grid.417009.b); the First Affiliated Hospital of Anhui Medical University, Department of Obstetrics and Gynecology, Reproductive Medicine Center, Anhui, China (GRID:grid.412679.f) (ISNI:0000 0004 1771 3402) 
2  China Agricultural University, College of Animal Science and Technology, Beijing, China (GRID:grid.22935.3f) (ISNI:0000 0004 0530 8290) 
3  Sun Yat-sen University, MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Guangzhou, China (GRID:grid.12981.33) (ISNI:0000 0001 2360 039X) 
4  The Third Affiliated Hospital of Guangzhou Medical University, Department of Obstetrics and Gynecology, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou, China (GRID:grid.417009.b) (ISNI:0000 0004 1758 4591); Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China (GRID:grid.417009.b) 
5  the First Affiliated Hospital of Anhui Medical University, Department of Obstetrics and Gynecology, Reproductive Medicine Center, Anhui, China (GRID:grid.412679.f) (ISNI:0000 0004 1771 3402) 
Pages
150
Publication year
2023
Publication date
Feb 2023
Publisher
Springer Nature B.V.
e-ISSN
20414889
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41419-023-05585-7
ProQuest document ID
2778775510
Copyright
© The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.