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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

The lack of viability of massive bone allografts for critical-size bone defect treatment remains a challenge in orthopedic surgery. The literature has reviewed the advantages of a multi-combined treatment with the synergy of an osteoconductive extracellular matrix (ECM), osteogenic stem cells, and growth factors (GFs). Questions are still open about the need for ECM components, the influence of the decellularization process on the latter, the related potential loss of function, and the necessity of using pre-differentiated cells. In order to fill in this gap, a bone allograft surrounded by an osteogenic membrane made of a decellularized collagen matrix from human fascia lata and seeded with periosteal mesenchymal stem cells (PMSCs) was analyzed in terms of de-/recellularization, osteogenic properties, PMSC self-differentiation, and angiogenic potential. While the decellularization processes altered the ECM content differently, the main GF content was decreased in soft tissues but relatively increased in hard bone tissues. The spontaneous osteogenic differentiation was necessarily obtained through contact with a mineralized bone matrix. Trying to deepen the knowledge on the complex matrix–cell interplay could further propel these tissue engineering concepts and lead us to provide the biological elements that allow bone integration in vivo.

Details

Title
A New Osteogenic Membrane to Enhance Bone Healing: At the Crossroads between the Periosteum, the Induced Membrane, and the Diamond Concept
Author
Manon, Julie 1   VIAFID ORCID Logo  ; Evrard, Robin 1 ; Fievé, Lies 2 ; Bouzin, Caroline 3   VIAFID ORCID Logo  ; Magnin, Delphine 4   VIAFID ORCID Logo  ; Xhema, Daela 5 ; Darius, Tom 6   VIAFID ORCID Logo  ; Bonaccorsi-Riani, Eliano 6 ; Gianello, Pierre 5 ; Pierre-Louis Docquier 7 ; Schubert, Thomas 8   VIAFID ORCID Logo  ; Lengelé, Benoît 9 ; Behets, Catherine 2   VIAFID ORCID Logo  ; Cornu, Olivier 8   VIAFID ORCID Logo 

 Morphology Lab (MORF), Institut de Recherche Expérimentale et Clinique (IREC), UCLouvain, 1200 Brussels, Belgium; Transplantation and Experimental Surgery Lab (CHEX), IREC, UCLouvain, 1200 Brussels, Belgium; Neuromusculoskeletal Lab (NMSK), IREC, UCLouvain, 1200 Brussels, Belgium; Centre de Thérapie Cellulaire et Tissulaire Locomoteur, Cliniques Universitaires Saint-Luc, 1200 Brussels, Belgium; Department of Orthopaedic and Trauma Surgery, Cliniques Universitaires Saint-Luc, 1200 Brussels, Belgium 
 Morphology Lab (MORF), Institut de Recherche Expérimentale et Clinique (IREC), UCLouvain, 1200 Brussels, Belgium 
 Imaging Platform (2IP), IREC, UCLouvain, 1200 Brussels, Belgium 
 Bio & Soft Matter (BSMA), Institute of Condensed Matter and Nanosciences (IMCN), UCLouvain, 1348 Louvain-la-Neuve, Belgium 
 Transplantation and Experimental Surgery Lab (CHEX), IREC, UCLouvain, 1200 Brussels, Belgium 
 Transplantation and Experimental Surgery Lab (CHEX), IREC, UCLouvain, 1200 Brussels, Belgium; Department of Abdominal Surgery and Transplantation, Cliniques Universitaires Saint-Luc, 1200 Brussels, Belgium 
 Neuromusculoskeletal Lab (NMSK), IREC, UCLouvain, 1200 Brussels, Belgium; Department of Orthopaedic and Trauma Surgery, Cliniques Universitaires Saint-Luc, 1200 Brussels, Belgium 
 Neuromusculoskeletal Lab (NMSK), IREC, UCLouvain, 1200 Brussels, Belgium; Centre de Thérapie Cellulaire et Tissulaire Locomoteur, Cliniques Universitaires Saint-Luc, 1200 Brussels, Belgium; Department of Orthopaedic and Trauma Surgery, Cliniques Universitaires Saint-Luc, 1200 Brussels, Belgium 
 Morphology Lab (MORF), Institut de Recherche Expérimentale et Clinique (IREC), UCLouvain, 1200 Brussels, Belgium; Department of Plastic and Reconstructive Surgery, Cliniques Universitaires Saint-Luc, UCLouvain, 1200 Brussels, Belgium 
First page
143
Publication year
2023
Publication date
2023
Publisher
MDPI AG
e-ISSN
23065354
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2779523698
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.