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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

(1) Background: Callerya atropurpurea is found in Laos, Thailand, and Vietnam. Although the anti-inflammatory action of C. atropurpurea has been investigated, the functions of this plant in allergic responses are not understood. Here, we explored the antiallergic mechanism of C. atropurpurea ethanol extract (Ca-EE) using in vitro assays and an in vivo atopic model. (2) Methods: The constituents of Ca-EE were analyzed using GC/MS. Inhibition of lipoxygenase and β-hexosaminidase activity was examined, and the expression of inflammatory genes was measured by quantitative real-time PCR. The regulatory roles of Ca-EE in IgE/FcεRI signaling were examined by Western blotting. The DNCB-induced atopic dermatitis mouse model was performed with histological analysis. (3) Results: Ca-EE comprised cis-raphasatin, lupeol, some sugars, and fatty acids. In RBL-2H3 cells, treatment with Ca-EE significantly reduced the activities of lipoxygenase and β-hexosaminidase, as well as cytokine gene expression. IgE-mediated signaling was downregulated by blocking Lyn kinases. Moreover, Ca-EE effectively inhibited allergic symptoms in the DNCB-induced atopic dermatitis model without toxicity. (4) Conclusions: Ca-EE displayed antiallergic activities through regulating IgE/Lyn signaling in RBL-2H3 cells and a contact dermatitis model. These results indicate that Ca-EE could be effective for allergic disease treatment.

Details

Title
Antiallergic Effects of Callerya atropurpurea Extract In Vitro and in an In Vivo Atopic Dermatitis Model
Author
Choi, Wooram 1 ; Lee, Hwa Pyoung 1 ; Manilack, Philaxay 2 ; Saysavanh, Veosavanh 2 ; Byoung-Hee, Lee 3   VIAFID ORCID Logo  ; Lee, Sarah 3 ; Kim, Eunji 4   VIAFID ORCID Logo  ; Cho, Jae Youl 1   VIAFID ORCID Logo 

 Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Republic of Korea 
 Department of Forestry, Ministry of Agriculture and Forestry, Vientiane P.O. Box 811, Laos 
 National Institute of Biological Resources, Environmental Research Complex, Incheon 22689, Republic of Korea 
 Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Republic of Korea; R&D Center, Yungjin Pharmaceutical Co., Ltd., Suwon 16229, Republic of Korea 
First page
860
Publication year
2023
Publication date
2023
Publisher
MDPI AG
e-ISSN
22237747
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2779674128
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.