Babesiosis acquired from a pet dog: a second reported case in Hong Kong

Abstract

Blood smear revealed the presence of intra-erythrocytic ring-form parasites, suggestive of Plasmodium falciparum infection (Fig 1). A case of human babesiosis acquired from a pet dog has been reported where the dog was heavily infested with ticks.5 In a Brazilian evaluation of ectoparasites in dogs kept in apartments, Ixodid nymphs were found in 2%. Recently a new Babesia species,Babesia hongkongensis has been identified in the feline population.10 The prevalence of this new local species is low among free-roaming cats in Hong Kong and the pathogenicity in pet cats is unknown. Complications of babesiosis include acute respiratory failure, disseminated intravascular coagulation, and multi-organ failure.3 Combination therapy with atovaquone and azithromycin is the treatment of choice for mild-to-moderate Babesia infection.

A 61-year-old Chinese man was admitted to our hospital in June 2012 with a 2-week history of fever and chills. After admission, he developed hypotension and respiratory distress and was transferred to the intensive care unit (ICU) for further management.

He frequently commuted between New York, Hong Kong, and Shanghai for business. He lived in his own house in New York and had a dog. Three weeks before onset of his symptoms, he swept his basement where his dog spent most of its time. He was not aware of any tick bite.

He was a chronic smoker but enjoyed good past health. His symptoms began with fever and chills while in China. He also experienced malaise and a mild dry cough. He had no skin rash, or abdominal or urinary symptoms. A diagnosis of upper respiratory tract infection was made and he was given a course of piperacillin-tazobactam during hospitalisation in China. Symptoms persisted, however, and he presented to a public hospital in Hong Kong. Initial blood tests revealed a normochromic normocytic anaemia (haemoglobin, 81 g/L) with reticulocytosis, marked thrombocytopenia, and moderate renal impairment. Blood smear revealed the presence of intra-erythrocytic ring-form parasites, suggestive of Plasmodium falciparum infection (Fig 1). Chest X-ray showed bilateral extensive abnormal lung infiltrates. He was transferred to our hospital for further management.

Figure 1. Intra-erythrocytic ring-form trophozoite in thinblood smear (Giemsa stain; original magnification, x 100)

He soon developed shock and was transferred to the ICU. Further blood tests revealed hyperbilirubinaemia and evidence of haemolysis. Peripheral blood smear for malaria was repeated and revealed 3.8% parasitised red cells. Pleomorphic ring forms were noted, with occasional arrangement in a cross-like pattern (Fig 2). Rapid antigen test for P falciparum was negative and this raised the suspicion of babesiosis. A subsequent blood Babesia microti polymerase chain reaction (PCR) test was positive and a diagnosis of babesiosis was confirmed.

Figure 2. Babesia microti in thin blood smear (Giemsa stain;original magnification, x 100). Tetrads of merozoites arearranged in a cross-like pattern (Maltese cross)

Quinine and clindamycin were initiated. The patient experienced severe tinnitus after 5 days of treatment and quinine was stopped. Doxycycline was added for treatment due to persistent fever and because it is known that Lyme's disease can be a concurrent infection. He stayed in the ICU for 6 days and his condition was stabilised with resolution of fever. Blood smear for Babesia turned negative on day 14 of treatment. Supportive blood transfusion was given for anaemia and the patient was discharged after 21 days.

He attended regular follow-ups in our clinic. Repeated blood smear for babesiosis and BabesiaPCR test (in September 2012, 3 months after treatment) were negative. He had the basement of his New York apartment professionally cleaned and had his dog treated for ticks.

Discussion

This is the second imported case of human babesiosis in Hong Kong since 2007.¹ Babesiosis is a tick-borne disease in which patients are infected with intra-erythrocytic parasites of the genus Babesia. The common species affecting humans are B microti andBabesia divergens, mostly found in the United States and Europe, respectively. White-footed mice are the primary reservoir host, but other small rodents may also carry B microti. The Babesia parasites are transmitted to humans accidentally by Ixodid tick bites. In the United States, Ixodes scapularis is the most common vector. The ticks become infected with B microti when they feed on vertebrate reservoir hosts such as infected white-footed mice and white-tailed deer. Humans are usually accidental hosts.²

The diagnosis of human babesiosis is made by microscopic examination of Giemsa-stained thick and thin blood smears. Microscopically, Babesiaspecies may appear as round or oval-shaped forms, with blue cytoplasm and red chromatin. Multiple parasites may be present in a single-infected red blood cell. Differentiation of the ring-form of Babesia species and P falciparum may be difficult. Some distinguishing features of babesiosis include the presence of extra-erythrocytic forms in severe cases and the absence of pigment deposits typically seen in older ring stages of P falciparum. The Maltese cross pattern in which tetrads of merozoites are arranged is pathognomonic of babesiosis, but they are not commonly seen.³ Nonetheless, the different species of babesiosis cannot be distinguished by microscopic examination. Real-time PCR performed on DNA targeting the 18S rRNA gene of B microtiis therefore a more specific and accurate method to detect B microti.⁴

Our patient was a case of possible pet-associated human Babesia infection. Babesiosis is a zoonotic protozoan disease of medical, veterinary, and economic importance. A case of human babesiosis acquired from a pet dog has been reported where the dog was heavily infested with ticks.⁵ In a Brazilian evaluation of ectoparasites in dogs kept in apartments, Ixodid nymphs were found in 2%. In houses with grassy yards, 18% of pet dogs were found to carry Ixodid nymphs.⁶ Different species of Ixodid ticks have been identified in dogs, with the head being the most common site of attachment. The activity of tick attachment peaks in spring and in autumn. Canine babesiosis is diagnosed by veterinarians in approximately 20% of tick-infested dogs.⁷ In one study in New York state, I scapularisticks were collected from recreational lands to determine the prevalence and distribution of tick-borne pathogens. The overall prevalence of B microtiwas approximately 3% among both nymphs and adult ticks.⁸

In Hong Kong, around 250 000 households are estimated to keep dogs or cats, representing 10.6% of all households.⁹ There has been no single reported case of human babesiosis in Hong Kong although the disease is common in the cat and dog population. Recently a new Babesia species,Babesia hongkongensis has been identified in the feline population.¹⁰ The prevalence of this new local species is low among free-roaming cats in Hong Kong and the pathogenicity in pet cats is unknown.

The clinical presentation of human babesiosis varies. Infected patients may present with a mild-to-moderate viral-like illness, with gradual onset of chills, sweats, headache, arthralgia, and anorexia. Some patients present with a prolonged course of pyrexia of unknown origin. Physical examination may reveal mild splenomegaly or hepatomegaly. Severe infection generally occurs in those with an underlying immunosuppressed condition, particularly patients with previous splenectomy. Complications of babesiosis include acute respiratory failure, disseminated intravascular coagulation, and multi-organ failure.³

Combination therapy with atovaquone and azithromycin is the treatment of choice for mild-to-moderate Babesia infection. In severe cases, the use of clindamycin and quinine is recommended. In general, the combination of atovaquone and azithromycin is better tolerated with fewer adverse effects.¹¹ All doses of antimicrobial therapy are administered for 7 to 10 days. For severely immunocompromised patients with persistent relapsing infection, a longer duration of at least 6 weeks' treatment is recommended, continuing for 2 weeks after blood smears become negative forBabesia. For fulminant cases, exchange transfusion may be required.^{2 12}

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