Abstract

Actinobacteria obtained from the least explored Indian regions were studied for their ability to suppress meningitis-causing bacteria in nanoparticle form. Drug-resistant bacteria and long-term treatment with different medications make meningitis control complicated. Thus, new meningitis drugs are required to combat MDR bacteria. In this study, secondary metabolites isolated from actinomycetes strains, Microbacterium proteolyticum LA2(R) and Streptomyces rochei LA2(O), were employed to synthesize silver nanoparticles (AgNPs) at 37 °C for seven days incubation. UV–Vis spectroscopy, TEM, FTIR, and HPLC studies were used for the confirmation of the synthesis of AgNPs. Furthermore, these NPs demonstrated antibacterial and antibiofilm activities against meningitis-causing bacteria. The average size of LA2(R) and LA2(O) isolated secondary metabolites mediated AgNPs was observed to be 27 ± 1and 29 ± 2 nm by TEM analysis. FTIR study of RAgNPs and OAgNPs revealed that presence of peaks with positions of 1637.17 cm1 and 1636.10 cm1 for C=O amide group appearances in the amide I linkage. These NPs were effective against bacterial pathogens such as S. pneumoniae, H. influenzae, and N. meningitidis and confirmed by their MICs, i.e., 109.4, 120.60, and 138.80 μg/ml of RAgNPs and 105.80, 114.40 and 129.06 μg/ml of OAgNPs, respectively. Additionally, the production of biofilms is impeded by these nanoparticles on S. pneumoniae, H. influenzae, and N. meningitidis by 73.14%, 71.89% and 64.81%, respectively. These findings confirm the potential role of synthesized AgNPs against biofilm forming meningitis causing Multidrug resistance (MDR) microbes.

Details

Title
Antibacterial efficacy of synthesized silver nanoparticles of Microbacterium proteolyticum LA2(R) and Streptomyces rochei LA2(O) against biofilm forming meningitis causing microbes
Author
Bano, Naushin 1 ; Iqbal, Danish 2 ; Al Othaim, Ayoub 3 ; Kamal, Mehnaz 4 ; Albadrani, Hind Muteb 3 ; Algehainy, Naseh A. 5 ; Alyenbaawi, Hadeel 3 ; Alghofaili, Fayez 3 ; Amir, Mohammad 1 ; Roohi 1   VIAFID ORCID Logo 

 Integral University, Protein Research Laboratory, Department of Bioengineering, Lucknow, India (GRID:grid.411723.2) (ISNI:0000 0004 1756 4240) 
 Buraydah Private Colleges, Department of Health Information Management, College of Applied Medical Sciences, Buraydah, Saudi Arabia (GRID:grid.411723.2) 
 Majmaah University, Department of Medical Laboratory Sciences, College of Applied Medical Science, Al-Majmaah, Saudi Arabia (GRID:grid.449051.d) (ISNI:0000 0004 0441 5633) 
 Prince Sattam Bin Abdulaziz University, Department of Pharmaceutical Chemistry, College of Pharmacy, Al-Kharj, Saudi Arabia (GRID:grid.449553.a) (ISNI:0000 0004 0441 5588) 
 University of Tabuk, Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Tabuk, Saudi Arabia (GRID:grid.440760.1) (ISNI:0000 0004 0419 5685) 
Pages
4150
Publication year
2023
Publication date
2023
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2786375383
Copyright
© The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.