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© Rael et al; licensee BioMed Central Ltd. 2009. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This work is published under http://creativecommons.org/licenses/by/2/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Background

In critical injury, the occurrence of increased oxidative stress or a reduced antioxidant status has been observed. The purpose of this study was to correlate the degree of oxidative stress, by measuring the oxidation-reduction potential (ORP) of plasma in the critically injured, with injury severity and serum amyloid A (SAA) levels.

Methods

A total of 140 subjects were included in this retrospective study comprising 3 groups: healthy volunteers (N = 21), mild to moderate trauma (ISS < 16, N = 41), and severe trauma (ISS ≥ 16, N = 78). For the trauma groups, plasma was collected on an almost daily basis during the course of hospitalization. ORP analysis was performed using a microelectrode, and ORP maxima were recorded for the trauma groups. SAA, a sensitive marker of inflammation in critical injury, was measured by liquid chromatography/mass spectrometry.

Results

ORP maxima were reached on day 3 (± 0.4 SEM) and day 5 (± 0.5 SEM) for the ISS < 16 and ISS ≥ 16 groups, respectively. ORP maxima were significantly higher in the ISS < 16 (-14.5 mV ± 2.5 SEM) and ISS ≥ 16 groups (-1.1 mV ± 2.3 SEM) compared to controls (-34.2 mV ± 2.6 SEM). Also, ORP maxima were significantly different between the trauma groups. SAA was significantly elevated in the ISS ≥ 16 group on the ORP maxima day compared to controls and the ISS < 16 group.

Conclusion

The results suggest the presence of an oxidative environment in the plasma of the critically injured as measured by ORP. More importantly, ORP can differentiate the degree of oxidative stress based on the severity of the trauma and degree of inflammation.

Details

Title
Injury severity and serum amyloid A correlate with plasma oxidation-reduction potential in multi-trauma patients: a retrospective analysis
Author
Rael, Leonard T 1 ; Bar-Or, Raphael 1 ; Salottolo, Kristin 1 ; Mains, Charles W 2 ; Slone, Denetta S 3 ; Offner, Patrick J 2 ; Bar-Or, David 4 

 Trauma Research, Swedish Medical Center, Englewood, (GRID:grid.416782.e) (ISNI:0000 0001 0503 5526); DMI Life Sciences, Inc, Greenwood Village, (GRID:grid.416782.e) 
 St Anthony Central Hospital, Trauma Services, Denver, (GRID:grid.490409.0) 
 Swedish Medical Center, Trauma Services, Englewood, (GRID:grid.416782.e) (ISNI:0000 0001 0503 5526) 
 Trauma Research, Swedish Medical Center, Englewood, (GRID:grid.416782.e) (ISNI:0000 0001 0503 5526); DMI Life Sciences, Inc, Greenwood Village, (GRID:grid.416782.e); Swedish Medical Center, Emergency Department, Englewood, (GRID:grid.416782.e) (ISNI:0000 0001 0503 5526); Rocky Vista University, Parker, (GRID:grid.461417.1) (ISNI:000000040445646X) 
Pages
57
Publication year
2009
Publication date
Dec 2009
Publisher
Springer Nature B.V.
ISSN
17577241
e-ISSN
15007480
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2788419909
Copyright
© Rael et al; licensee BioMed Central Ltd. 2009. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This work is published under http://creativecommons.org/licenses/by/2/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.