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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Colistin is a last-resort antibiotic used to treat infections caused by multidrug-resistant Gram-negative bacteria. People with a history of travel to the Dominican Republic have become sick with pathogenic bacteria carrying the mobile colistin resistance gene, mcr-1, during and after traveling. This investigation aimed to identify mcr genes in Enterobacteriaceae isolated from food animal sources in the Dominican Republic. Three hundred and eleven samples were tested, from which 1354 bacterial isolates were obtained. Real-time PCR tests showed that 70.7% (220 out of 311) of the samples and 3.2% (44 out of 1354) of the isolates tested positive for the mcr gene. All RT-PCR presumptive mcr-positive isolates (n = 44) and a subset (n = 133) of RT-PCR presumptive mcr-negative isolates were subjected to whole-genome sequencing. WGS analysis showed that 39 isolates carried the mcr gene, with 37 confirmed as positive through RT-PCR and two as negative. Further, all of the mcr-positive genomes were identified as Escherichia coli and all contained a IncX4 plasmid replicon. Resistant determinants for other antibiotics important for human health were found in almost all isolates carrying mcr genes.

Details

Title
First Known Report of mcr-Harboring Enterobacteriaceae in the Dominican Republic
Author
Perdomo, Angela 1   VIAFID ORCID Logo  ; Webb, Hattie E 2   VIAFID ORCID Logo  ; Bugarel, Marie 3 ; Friedman, Cindy R 4   VIAFID ORCID Logo  ; Watkins, Louise K Francois 2   VIAFID ORCID Logo  ; Loneragan, Guy H 1   VIAFID ORCID Logo  ; Calle, Alexandra 1   VIAFID ORCID Logo 

 School of Veterinary Medicine, Texas Tech University, Amarillo, TX 79409, USA 
 Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA 
 Division of Research and Development Resources, BioMérieux, 69795 Lyon, France 
 Division of Global Migration and Quarantine, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA 
First page
5123
Publication year
2023
Publication date
2023
Publisher
MDPI AG
ISSN
1661-7827
e-ISSN
1660-4601
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2791652238
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.